alterlab-scvelo

What problem does it solve?

RNA velocity analysis provides a framework to infer cell state transitions in single-cell RNA-seq by comparing unspliced and spliced mRNA to estimate transcriptional dynamics and trajectories.

Core Features & Use Cases

• Trajectory inference from snapshot scRNA-seq data using splicing kinetics
• Latent time estimation and downstream visualization on embeddings (UMAP)
• Driver gene analysis via velocity-based metrics to identify regulators
• Complement to Scanpy integration for rich, end-to-end analyses
• Real-world example: map developmental trajectories across heterogeneous cell populations using scVelo

Quick Start

Run the scVelo workflow on your AnnData object to compute velocity and latent time.