bamtofastq

Convert BAM or CRAM inputs into FASTQ outputs for nf-core bamtofastq runs.

1|Updated Jun 19, 2026
One-click install
npx skills add https://github.com/danilomonge/nf-claw --skill bamtofastq
Or copy as Structured Prompt for Agent
Please help me install this Agent Skill.
Skill: bamtofastq
Source: https://github.com/danilomonge/nf-claw/tree/main/pipelines/bamtofastq
Command: npx skills add https://github.com/danilomonge/nf-claw --skill bamtofastq

SYSTEM DOCUMENTATION & REQUIREMENTS

What problem does it solve?

This Skill removes the guesswork from running nf-core bamtofastq by turning a pinned bioinformatics workflow into a validated, reproducible, and agent-friendly execution plan.

Core Features & Use Cases

  • Fastq Conversion Workflow: Converts BAM or CRAM inputs into FASTQ outputs for downstream analysis.
  • Release-Aware Execution: Supports pinned pipeline releases, Nextflow version selection, and on-demand release documentation.
  • Production Guardrails: Enforces samplesheet structure, required parameters, allowed values, and provenance capture for safer runs.
  • Use Case: A researcher needs to convert many sequencing alignments into FASTQ files and rerun an older release with a specific Nextflow engine version for compatibility.

Quick Start

Ask the bamtofastq skill to run the pipeline for your samplesheet, output directory, and preferred environment profile.

Frequently Asked Questions about bamtofastq

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I convert BAM or CRAM files to FASTQ using Nextflow?

To convert BAM or CRAM files to FASTQ, this Skill executes the nf-core bamtofastq pipeline using a validated samplesheet, output directory, and environment profile to ensure reproducible FASTQ extraction.

What is the best way to run a pinned nf-core bamtofastq release with a specific Nextflow version?

Running a pinned nf-core bamtofastq release requires selecting a specific Nextflow engine version and applying release-specific documentation to maintain compatibility and capture provenance during execution.

Can I extract FASTQ reads from BAM files restricted to specific chromosomes or regions?

Yes, you can extract FASTQ reads from BAM inputs by restricting the conversion to specific chromosomes or regions, driven by a validated samplesheet and schema-checked pipeline flags.

Does the nf-core bamtofastq workflow enforce samplesheet validation before running?

The nf-core bamtofastq workflow enforces production guardrails by validating samplesheet structure, required parameters, and allowed values before executing the BAM to FASTQ conversion.

Why do I need a validated samplesheet for Nextflow bioinformatics jobs?

A validated samplesheet is required for Nextflow bioinformatics jobs to enforce correct structure, required parameters, and provenance-aware execution, preventing pipeline failures during BAM to FASTQ conversion.