bio-crispr-screens-screen-qc
OfficialQC CRISPR screens: fast quality checks.
Authorstellaromics
Version1.0.0
Installs0
System Documentation
What problem does it solve?
Quality control for pooled CRISPR screens, enabling quick assessment of library representation, read distribution, replicate concordance, and essential gene recovery to ensure reliable hit calling.
Core Features & Use Cases
- Library Representation: Evaluate sgRNA coverage across samples to flag underrepresented libraries.
- Read Distribution & Gini: Quantify uniform read distribution to identify skew and ensure data quality.
- Replicate Correlation: Measure and visualize reproducibility between replicates to detect batch effects.
- Essential Gene Recovery: Validate screen performance against known essential genes using AUROC or related metrics.
- Use Case: Apply QC before hit calling to decide if the experiment should be re-run or include more replicates.
Quick Start
Run the QC workflow on your MAGeCK-derived results to obtain a concise report and diagnostics.
Dependency Matrix
Required Modules
None requiredComponents
Standard package💻 Claude Code Installation
Recommended: Let Claude install automatically. Simply copy and paste the text below to Claude Code.
Please help me install this Skill: Name: bio-crispr-screens-screen-qc Download link: https://github.com/stellaromics/fast-bioinfo/archive/main.zip#bio-crispr-screens-screen-qc Please download this .zip file, extract it, and install it in the .claude/skills/ directory.
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