bio-de-visualization

Generate MA plots, heatmaps, and PCA from DESeq2 or edgeR results.

Updated Mar 30, 2026
One-click install
npx skills add https://github.com/ya-way/cytoclaw-skills --skill bio-de-visualization
Or copy as Structured Prompt for Agent
Please help me install this Agent Skill.
Skill: bio-de-visualization
Source: https://github.com/ya-way/cytoclaw-skills/tree/main/workspace/skills/bio-differential-expression-de-visualization
Command: npx skills add https://github.com/ya-way/cytoclaw-skills --skill bio-de-visualization

SYSTEM DOCUMENTATION & REQUIREMENTS

What problem does it solve?

Differential expression analysis in RNA-seq often produces complex results that are hard to interpret from raw tables alone. This Skill provides ready-to-use visualizations (MA plots, PCA, heatmaps, dispersion plots, and counts plots) to help researchers quickly understand DE patterns and communicate findings.

Core Features & Use Cases

  • Generate MA plots, heatmaps, PCA plots, dispersion plots, and counts plots from DESeq2 or edgeR outputs.
  • Support common RNA-seq differential analysis workflows, including identifying top DE genes and comparing multiple conditions across samples.
  • Provide guidance on interpreting visuals and troubleshooting plotting issues for robust data exploration.

Quick Start

Generate a PCA plot of your samples colored by condition from your DESeq2 results.

Frequently Asked Questions about bio-de-visualization

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I generate publication-ready plots from DESeq2 or edgeR RNA-seq results?

To generate publication-ready plots from DESeq2 or edgeR RNA-seq results, use this Skill to create MA plots, heatmaps, PCA plots, and dispersion plots. It provides an end-to-end workflow using R libraries like DESeq2, ggplot2, and pheatmap.

What types of RNA-seq differential expression visualizations can I create for multiple conditions?

For RNA-seq differential expression visualizations across multiple conditions, you can create MA plots, heatmaps, PCA plots, dispersion plots, and counts plots. These visuals help identify top DE genes and compare per-sample expression patterns.

Can I visualize top differentially expressed genes in RNA-seq data using a heatmap?

Yes, you can visualize top differentially expressed genes in RNA-seq data using heatmaps. The Skill uses the pheatmap R library to generate per-sample expression visuals, helping you quickly understand and communicate DE patterns from your analysis.

Does this RNA-seq visualization workflow support PCA plots colored by sample condition?

Yes, this RNA-seq visualization workflow supports generating PCA plots colored by sample condition. It includes preprocessing steps and plotting functions to compare multiple conditions across samples directly from DESeq2 or edgeR outputs.

What do I need to plot RNA-seq differential expression results without manual ggplot2 coding?

To plot RNA-seq differential expression results without manual coding, you need outputs from DESeq2 or edgeR analysis. The Skill handles preprocessing and uses R libraries like ggplot2 and pheatmap internally to produce saved visual outputs automatically.

Why are my RNA-seq DESeq2 plots not displaying differential expression patterns clearly?

If your RNA-seq DESeq2 plots are not displaying clearly, this Skill provides guidance on interpreting visuals and troubleshooting plotting issues. It ensures robust data exploration by applying proper preprocessing steps before generating MA plots and heatmaps.