bio-workflows-multiome-pipeline

What problem does it solve?

End-to-end multiome workflow for joint scRNA-seq and scATAC-seq analysis, enabling streamlined data loading, QC, modality processing, and integrated interpretation.

Core Features & Use Cases

• Load 10X Multiome outputs and create a unified Seurat object with RNA and ATAC assays.
• Independently QC and preprocess RNA and ATAC modalities, then integrate them via Weighted Nearest Neighbors (WNN) for joint clustering and visualization.
• Generate linked features to explore regulatory relationships and multi-omics drivers across cell types.
• Use cases include discovering cell types with combined RNA/ATAC signals and comparing modality contributions per cluster.

Quick Start

Load your 10X Multiome output and run the end-to-end Seurat/Signac pipeline to generate integrated RNA and ATAC analyses.