bio-workflows-riboseq-pipeline

What problem does it solve?

End-to-end ribosome profiling data analysis from FASTQ to translation efficiency and ORF detection, enabling researchers to extract TE and identify translated ORFs in a streamlined workflow.

Core Features & Use Cases

• Preprocessing: adapter trimming and quality filtering of raw reads.
• Alignment and P-site calibration: map reads to transcriptome and calibrate P-site offsets for accurate TE estimation.
• Translation efficiency & ORF detection: compute TE and call translated ORFs (RiboCode) across experiments.
• Real-world use: process a ribosome profiling dataset from FASTQ through TE and ORF reporting to publishable results.

Quick Start

Run the complete ribosome profiling pipeline from FASTQ to translation efficiency and ORF detection.