bioinformatics-init-analysis

Automate single-cell data analysis from loading through HTML report generation.

Updated Aug 27, 2026
One-click install
npx skills add https://github.com/unstun/dqn10 --skill bioinformatics-init-analysis-unstun
Or copy as Structured Prompt for Agent
Please help me install this Agent Skill.
Skill: bioinformatics-init-analysis
Source: https://github.com/unstun/dqn10/tree/main/.claude/skills/bioinformatics-init-analysis
Command: npx skills add https://github.com/unstun/dqn10 --skill bioinformatics-init-analysis-unstun

SYSTEM DOCUMENTATION & REQUIREMENTS

💡 This Skill requires numpy, pandas, scanpy, anndata, fcsparser, scikit-learn, and includes scripts (resource) and references (resource) components.

What problem does it solve?

Automates a full end-to-end analysis pipeline for high-dimensional single-cell biology data, enabling researchers to process CyTOF, scRNA-seq, and flow cytometry with a single pipeline.

Core Features & Use Cases

  • 7-step pipeline: Load → QC → Normalize → PCA/UMAP → Cluster → Marker Analysis → Report
  • Auto-detection of data type with support for CyTOF, scRNA-seq, and flow cytometry files
  • Clinical/plain-language reports and modular execution (full pipeline or individual steps)
  • Real-world use: researchers can run the entire pipeline on a dataset and generate a ready-to-read clinical report

Quick Start

Install the Claude Code plugin and run the full pipeline on your dataset with an input path to auto-detect data type and generate a clinical report.

Frequently Asked Questions about bioinformatics-init-analysis

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I automate scRNA-seq and flow cytometry analysis in a single pipeline?

You can automate single-cell data analysis by running a 7-step pipeline that loads data, performs QC and normalization, applies PCA/UMAP, clusters cells, and runs marker analysis to output a processed dataset and clinical HTML report.

Can I run individual steps like clustering and marker analysis separately?

Yes, the pipeline supports modular execution, allowing you to run individual steps like clustering or marker analysis independently rather than executing the full end-to-end pipeline at once.

Does this single-cell pipeline support CyTOF and flow cytometry data formats?

Yes, the pipeline auto-detects data types and fully supports high-dimensional single-cell biology workflows including CyTOF, scRNA-seq, and flow cytometry files.

How does auto-detection work for scRNA-seq and flow cytometry files?

The pipeline automatically detects your single-cell data type by evaluating the input path, applying the appropriate processing logic for scRNA-seq, CyTOF, or flow cytometry without requiring manual configuration.

What is the best way to generate clinical reports from high-dimensional cytometry data?

The best way is to use an end-to-end pipeline that processes high-dimensional single-cell data and automatically generates a plain-language HTML clinical report alongside the processed dataset.

Do I need scanpy and anndata installed to process scRNA-seq data?

Yes, the pipeline requires scanpy, anndata, numpy, pandas, scikit-learn, and fcsparser to handle data loading, normalization, dimensionality reduction, and clustering across single-cell formats.