design-prime-editor-sgrnas
CommunityDesign pegRNAs for prime editing guides.
AuthorMDhewei
Version1.0.0
Installs0
System Documentation
What problem does it solve?
Design pegRNAs (pegRNAs) and nicking sgRNAs for prime editing to enable precise genomic edits with reduced double-strand breaks. Given a target gene and a desired edit, this tool automates identification of PE-compatible protospacers, design of reverse-transcriptase templates and PBS sequences, and scoring of predicted guide efficiency.
Core Features & Use Cases
- Identify PE-compatible protospacers near the edit site on both DNA strands with customizable PAMs.
- Design PBS and RT templates and compute PBS melting temperature, followed by efficiency scoring; for PE3, nicking sgRNA candidates are also identified.
- Output includes a TSV with spacer, PBS, RT template, scores, and optional nicking guides, plus plots for distributions and context visualization.
Quick Start
Supply a gene symbol (or explicit sequence) and the desired edit to receive ranked pegRNA designs and nicking sgRNA options.
Dependency Matrix
Required Modules
requestsnumpypandasmatplotlib
Components
scripts
💻 Claude Code Installation
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Please help me install this Skill: Name: design-prime-editor-sgrnas Download link: https://github.com/MDhewei/bioinfor-claw/archive/main.zip#design-prime-editor-sgrnas Please download this .zip file, extract it, and install it in the .claude/skills/ directory.
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