em-expert

What problem does it solve?

EM methods writing and tool selection often blur critical modality and pipeline distinctions, causing incorrect guidance about how data should be acquired, preprocessed, segmented, reconstructed, and interpreted.

Core Features & Use Cases

• Modality clarity across the EM landscape: correctly distinguishes TEM vs SEM, SPA vs cryo-ET, resin/plastic-section EM vs volume EM, and explains what each acronym really means for the downstream pipeline.
• Stage-by-stage pipeline guidance: maps typical EM workflow stages (acquisition → motion/CTF → picking/segmentation → alignment/reconstruction → post-processing → interpretation) to the kinds of tools that fit each stage and why.
• Cross-modality and CLEM/cyro-FIB lift-out accuracy: explains CLEM vs cryo-CLEM differences, correlation constraints, fiducials/registration accuracy, and lamella prep effects that change achievable outcomes.

Quick Start

Use the em-expert skill when drafting or revising an EM methods section to ensure each modality and pipeline stage uses accurate, non-interchangeable terminology and appropriate tool-to-task matching.