hgtseq

Run the nf-core hgtseq pipeline from a samplesheet with validated parameters.

1|Updated Jun 19, 2026
One-click install
npx skills add https://github.com/danilomonge/nf-claw --skill hgtseq
Or copy as Structured Prompt for Agent
Please help me install this Agent Skill.
Skill: hgtseq
Source: https://github.com/danilomonge/nf-claw/tree/main/pipelines/hgtseq
Command: npx skills add https://github.com/danilomonge/nf-claw --skill hgtseq

SYSTEM DOCUMENTATION & REQUIREMENTS

What problem does it solve?

nf-core hgtseq automates a complex horizontal gene transfer analysis workflow so researchers do not have to manually stitch together preprocessing, alignment, taxonomic screening, and reporting steps.

Core Features & Use Cases

  • Reproducible release-pinned execution of the hgtseq pipeline.
  • Validated sample sheet and required parameters for reliable runs.
  • Built-in output organization, MultiQC reporting, and provenance capture.
  • Use case: a bioinformatics researcher can analyze sequencing data for suspected horizontal gene transfer and generate a standardized results directory for review and sharing.

Quick Start

Ask the agent to run hgtseq on my samplesheet with the required taxonomy ID, output directory, and reference databases, then produce the pipeline results and report.

Frequently Asked Questions about hgtseq

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I run the nf-core hgtseq pipeline for horizontal gene transfer analysis?

To run the hgtseq pipeline, provide a validated samplesheet, required taxonomy ID, reference databases, and output directory. The Skill executes the nf-core workflow, validates inputs, and generates standardized results with MultiQC reporting.

What is horizontal gene transfer analysis in next-generation sequencing data?

Horizontal gene transfer analysis identifies genetic material movement between organisms using next-generation sequencing data. The nf-core hgtseq pipeline automates preprocessing, alignment, taxonomic screening, and reporting steps into a reproducible workflow.

How do I reproduce a previous hgtseq pipeline run with the same version?

The Skill supports release-pinned reruns by fixing the selected nf-core hgtseq pipeline version and recording runtime environment provenance. This captures the exact execution context needed to reproduce results consistently.

Can I use the Nextflow hgtseq pipeline in a containerized environment?

Yes, the Skill supports reproducible execution on both local and containerized Nextflow setups. It records provenance for the selected pipeline version and runtime environment to ensure consistent results across different configurations.

Do I need to manually validate my samplesheet before running the hgtseq pipeline?

No, the Skill automatically validates the samplesheet and required parameters before execution. It enforces schema-constrained flags and allowed values, ensuring inputs meet the pipeline requirements without manual preprocessing.