init-analysis

Automate seven-step single-cell analysis with QC, clustering, and report generation.

Updated Apr 1, 2026
One-click install
npx skills add https://github.com/xiaoyu-ops/the_work_of_dedup --skill init-analysis-xiaoyu-ops
Or copy as Structured Prompt for Agent
Please help me install this Agent Skill.
Skill: init-analysis
Source: https://github.com/xiaoyu-ops/the_work_of_dedup/tree/main/.claude/skills/bioinformatics-init-analysis/skills/init-analysis
Command: npx skills add https://github.com/xiaoyu-ops/the_work_of_dedup --skill init-analysis-xiaoyu-ops

SYSTEM DOCUMENTATION & REQUIREMENTS

💡 This Skill includes scripts (resource) and references (resource) components.

What problem does it solve?

Researchers often need to perform complex, multi-step analyses on high-dimensional single-cell data (scRNA-seq, CyTOF, flow cytometry) and generate interpretable reports. This skill provides an automated, reproducible 7-step pipeline that handles data loading, QC, normalization, dimensionality reduction, clustering, marker analysis, and reporting, saving time and reducing user-driven variability.

Core Features & Use Cases

  • Auto-detects data type (scRNA-seq, CyTOF, flow) and adapts processing.
  • Modular 7-step pipeline: data loading, QC, normalization, dimensionality reduction, clustering, marker analysis, and report generation.
  • Produces plain-language reports and an HTML output suitable for researchers, clinicians, or data scientists performing exploratory analyses.

Quick Start

Run the pipeline on your single-cell dataset to generate a plain-language report, processed data, and figures.

Frequently Asked Questions about init-analysis

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I automate scRNA-seq analysis from raw data to clustering and marker analysis?

You can automate scRNA-seq analysis by running a modular 7-step pipeline that handles data loading, QC, normalization, dimensionality reduction, clustering, and marker analysis automatically. The pipeline detects your data type and outputs an interpretable HTML report with processed data and figures.

What does a standard single-cell QC and normalization workflow include for high-dimensional data?

A standard single-cell QC and normalization workflow includes data loading, quality control filtering, normalization, dimensionality reduction, clustering, marker analysis, and report generation. This pipeline supports scRNA-seq, CyTOF, and flow cytometry data, producing plain-language reports for downstream interpretation.

Can I use the same pipeline for both scRNA-seq and CyTOF data without changing parameters?

Yes, the pipeline auto-detects whether your input is scRNA-seq, CyTOF, or flow cytometry data and adapts its processing accordingly. You provide raw datasets or folders, and the workflow automatically configures the appropriate QC, normalization, and analysis steps for each data type.

What's the best way to run marker analysis and dimensionality reduction on flow cytometry data?

The best way to run marker analysis and dimensionality reduction on flow cytometry data is using an automated pipeline that detects the data type and applies a modular 7-step workflow. This produces an HTML report with clustering results and marker findings suitable for exploratory analysis.

Do I need to manually separate scRNA-seq and flow cytometry files before running single-cell analysis?

No, you do not need to manually separate scRNA-seq and flow cytometry files before analysis. The pipeline automatically detects the data type from your raw datasets or folders and adapts the processing steps accordingly, handling scRNA-seq, CyTOF, and flow cytometry without manual intervention.