isoseq

What problem does it solve?

This Skill removes the guesswork from running the nf-core/isoseq pipeline by giving you the exact inputs, required parameters, output expectations, and release-specific guidance needed to launch a correct analysis on the first try.

Core Features & Use Cases

• Validated Pipeline Execution: Run the pinned latest release or a specific version with the correct command structure and parameter set.
• Sample Sheet Driven Inputs: Use the required CSV samplesheet format to define sequencing inputs cleanly and consistently.
• Clear Output and Provenance: Understand where results, pipeline reports, and run metadata are written so analyses are reproducible.
• Research Use Case: Use this Skill when processing Isoseq subreads for gene and transcript annotation with the right aligner, primers, and version controls.

Quick Start

Use the isoseq skill to run the nf-core/isoseq pipeline on my samplesheet with the required primers and aligner settings.