lsmquant

Run the nf-core/lsmquant pipeline for light-sheet microscopy image preprocessing and analysis.

1|Updated Jun 19, 2026
One-click install
npx skills add https://github.com/danilomonge/nf-claw --skill lsmquant
Or copy as Structured Prompt for Agent
Please help me install this Agent Skill.
Skill: lsmquant
Source: https://github.com/danilomonge/nf-claw/tree/main/pipelines/lsmquant
Command: npx skills add https://github.com/danilomonge/nf-claw --skill lsmquant

SYSTEM DOCUMENTATION & REQUIREMENTS

💡 This Skill includes references (resource) components.

What problem does it solve?

It helps researchers run the nf-core/lsmquant workflow without guessing flags, manually checking schema files, or drifting from the pinned release.

Core Features & Use Cases

  • Pinned pipeline execution: Run the exact lsmquant release bundled in the repository for reproducible results.
  • Validated inputs and parameters: Work from a samplesheet and schema-backed options so invalid values fail fast.
  • Clear outputs and provenance: Produce module results, pipeline info, and run logs suitable for repeatable bioinformatics analysis.
  • Use Case: A microscopy lab needs to preprocess tissue-clearing images, perform stitching or alignment, and generate quantification outputs from one standardized command.

Quick Start

Use the lsmquant skill to run the pinned pipeline on your samplesheet and save the results to your chosen output directory.

Frequently Asked Questions about lsmquant

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I run a light-sheet microscopy pipeline for nuclei quantification?

Run the nf-core/lsmquant pipeline by providing a valid input samplesheet CSV, an output directory, and schema-constrained parameters to execute preprocessing, stitching, alignment, registration, and nuclei quantification for tissue-clearing imaging workflows.

What inputs do I need for nf-core light-sheet microscopy image analysis?

Light-sheet microscopy image analysis requires a valid input CSV samplesheet, an output directory, and schema-constrained parameters aligned with the pinned pipeline release and Nextflow runtime to process tissue-clearing imaging data.

Can I use this pipeline for tissue-clearing image stitching and alignment?

Yes, the pipeline applies to tissue-clearing imaging workflows that require staged stitching, alignment, registration, and nuclei quantification, starting from a standardized samplesheet input.

How does schema validation work for Nextflow microscopy pipeline parameters?

Schema validation checks parameters against the pinned pipeline release schema, ensuring invalid values fail fast before execution. This prevents parameter drift and enforces reproducible bioinformatics analysis runs.

What's the best way to ensure reproducible light-sheet microscopy quantification results?

Use a pinned pipeline release bundled in the repository with schema-backed options and validated inputs. This produces module results, pipeline info, and run logs suitable for repeatable bioinformatics analysis.

Do I need Nextflow installed to run the lsmquant microscopy pipeline?

Yes, the pipeline requires the Nextflow runtime and schema-constrained parameters aligned with the pinned pipeline release to execute light-sheet microscopy preprocessing and nuclei quantification workflows.