nextflow-development

Automate nf-core bioinformatics pipeline execution for omics data analysis.

Updated Mar 5, 2026
One-click install
npx skills add https://github.com/8GG-Git/knowledge-work-plugins --skill nextflow-development
Or copy as Structured Prompt for Agent
Please help me install this Agent Skill.
Skill: nextflow-development
Source: https://github.com/8GG-Git/knowledge-work-plugins/tree/main/bio-research/skills/nextflow-development
Command: npx skills add https://github.com/8GG-Git/knowledge-work-plugins --skill nextflow-development

SYSTEM DOCUMENTATION & REQUIREMENTS

💡 This Skill includes scripts (resource) and references (resource) components.

What problem does it solve?

This Skill automates the execution of complex bioinformatics pipelines (like RNA-seq, variant calling, and ATAC-seq analysis) directly within Claude, eliminating the need for manual command-line execution and environment setup.

Core Features & Use Cases

  • Pipeline Execution: Run nf-core pipelines (rnaseq, sarek, atacseq) for omics data analysis.
  • Environment Setup: Guides users through installing and verifying necessary tools (Docker, Nextflow, Java).
  • Data Preparation: Assists in downloading public data from GEO/SRA and generating compatible samplesheets.
  • Use Case: A researcher can provide a GEO accession number, and Claude will download the relevant RNA-seq data, set up the environment, and run the nf-core/rnaseq pipeline to generate gene expression counts.

Quick Start

Use the nextflow-development skill to analyze the RNA-seq data from GEO accession GSE110004.

Frequently Asked Questions about nextflow-development

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I run nf-core RNA-seq pipelines using only a GEO accession number?

Run nf-core RNA-seq pipelines by providing a GEO accession number. The tool downloads public data from GEO/SRA, generates compatible samplesheets, configures the environment, and executes the nf-core/rnaseq pipeline automatically.

What do I need to install to execute Nextflow bioinformatics pipelines locally?

To execute Nextflow bioinformatics pipelines, you need Docker, Java, and Nextflow installed. The tool guides you through verifying these dependencies and setting up the required environment before running nf-core workflows.

Can I use this to automate variant calling for WGS and WES data?

Yes, you can automate variant calling for WGS and WES data. The tool executes the nf-core/sarek pipeline directly, handling the complex command-line execution and environment setup required for genomic variant analysis.

How do I prepare SRA data for ATAC-seq analysis without manual command-line formatting?

Prepare SRA data for ATAC-seq analysis by letting the tool acquire public datasets and generate compatible samplesheets automatically. It then executes the nf-core/atacseq pipeline using Nextflow and Docker without manual formatting.

Is Docker required to run nf-core pipelines like sarek and atacseq?

Docker is required to run nf-core pipelines like sarek and atacseq. The tool relies on Docker containers alongside Nextflow and Java to ensure consistent environments for omics data analysis.

What is the best way to generate samplesheets for nf-core omics data analysis?

The best way to generate samplesheets for nf-core omics data analysis is to let the tool create them automatically. It formats downloaded public data from GEO/SRA into compatible inputs for pipelines like rnaseq, sarek, and atacseq.