omics-scrna

Execute single-cell RNA-seq workflows with QC, batch integration, and cell-type annotation.

Updated Aug 27, 2026
One-click install
npx skills add https://github.com/Minions-Land/AutOmicScience --skill omics-scrna
Or copy as Structured Prompt for Agent
Please help me install this Agent Skill.
Skill: omics-scrna
Source: https://github.com/Minions-Land/AutOmicScience/tree/main/skills/omics/scrna
Command: npx skills add https://github.com/Minions-Land/AutOmicScience --skill omics-scrna

SYSTEM DOCUMENTATION & REQUIREMENTS

💡 This Skill includes references (resource) components.

What problem does it solve?

This Skill solves the challenge of reproducible bioinformatics analysis by providing an autonomous, evidence-based framework for single-cell RNA-seq processing that eliminates hallucinated results and manual pipeline errors.

Core Features & Use Cases

  • Automated Pipeline Execution: Orchestrates QC, normalization, batch integration, and clustering using pinned scverse environments.
  • Grounded Annotation: Performs cell-type labeling using marker-based evidence and LLM reasoning, with built-in abstention for ambiguous clusters.
  • Use Case: A researcher needs to process a 10x scRNA-seq dataset from raw counts to annotated cell types; this Skill executes the workflow, records tool-call evidence for every step, and validates the biological integrity of the results.

Quick Start

Run the omics-scrna skill to perform quality control and preprocessing on the raw h5ad file named raw_data.h5ad.

Frequently Asked Questions about omics-scrna

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I automate single-cell RNA-seq analysis from raw counts to cell-type annotation?

Automate single-cell RNA-seq analysis by running autonomous workflows for QC, normalization, batch integration, and clustering using pinned scverse environments. The pipeline processes raw h5ad files and performs marker-based cell-type annotation with evidence-backed biological validation.

What is the best way to perform reproducible scRNA-seq batch integration using Scanpy?

Reproducible scRNA-seq batch integration uses a pinned Pixi environment and the omics_runtime tool registry for deterministic tool dispatch. This eliminates manual pipeline errors by recording tool-call evidence for every step executed within the scverse ecosystem.

Can I use this scverse pipeline to annotate ambiguous cell clusters automatically?

The scverse pipeline performs cell-type labeling using marker-based evidence and LLM reasoning. It includes built-in abstention for ambiguous clusters, ensuring that only biologically validated and evidence-backed annotations are returned for your single-cell dataset.

Do I need a specific environment setup to run autonomous bioinformatics workflows for scRNA-seq?

You need a pinned Pixi environment and access to the omics_runtime tool registry for deterministic tool dispatch. This setup ensures reproducible scientific compute by enforcing a strict scverse ecosystem configuration for your scRNA-seq data processing.

Why does my scRNA-seq pipeline return hallucinated cell types and manual errors?

Hallucinated results and manual pipeline errors occur without an evidence-based framework. This Skill eliminates them by orchestrating autonomous scverse workflows and recording tool-call evidence for every QC, integration, and annotation step to validate biological integrity.

What are the limitations of using autonomous workflows for scRNA-seq quality control?

Autonomous scRNA-seq quality control workflows require a pinned Pixi environment and omics_runtime tool registry access. Without these deterministic configurations, the scverse-based pipeline cannot guarantee reproducible tool dispatch or evidence-backed scientific compute for multi-agent research environments.