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opentrons-integration

Develop and execute Opentrons liquid handling protocols using the Protocol API v2.

8|Updated Nov 19, 2025
Search Tags:#robotics#pipetting#lab automation#opentrons#protocol development#liquid handling
One-click install
npx skills add https://github.com/sanand0/scientific-research --skill opentrons-integration-sanand0
Or copy as Structured Prompt for Agent▼
Please help me install this Agent Skill.
Skill: opentrons-integration
Source: https://github.com/sanand0/scientific-research/tree/main/.claude/skills/opentrons-integration
Command: npx skills add https://github.com/sanand0/scientific-research --skill opentrons-integration-sanand0

SYSTEM DOCUMENTATION & REQUIREMENTS

💡 This Skill includes scripts (resource) and references (resource) components.

What problem does it solve?

This Skill automates complex liquid handling tasks in a laboratory setting, enabling precise and repeatable pipetting workflows for scientific research and development.

Core Features & Use Cases

  • Protocol Development: Write and execute Python-based protocols for Opentrons robots (Flex/OT-2).
  • Hardware Control: Manage pipettes, labware, and modules (temperature, magnetic, thermocycler, heater-shaker).
  • Use Case: Automate the setup of PCR reactions, including master mix distribution and sample addition, followed by thermocycling.

Quick Start

Use the opentrons-integration skill to write a protocol that transfers 100µL of liquid from source plate well A1 to destination plate well B1.

Frequently Asked Questions about opentrons-integration

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I automate PCR setup and liquid handling workflows using Opentrons robots?▼

You can automate PCR setup and liquid handling workflows by writing Python-based protocols using the Opentrons Protocol API v2. This enables precise pipetting, master mix distribution, and thermocycler control for Flex and OT-2 robots.

What is Opentrons Protocol API v2 used for in lab automation?▼

The Opentrons Protocol API v2 is used for developing and executing automated laboratory liquid handling protocols. It facilitates precise pipetting, labware management, and hardware module control for Flex and OT-2 robots.

Can I use Python to control temperature and magnetic modules on OT-2 robots?▼

Yes, Python protocols can manage hardware modules including temperature, magnetic, thermocycler, and heater-shaker modules on OT-2 robots. This allows for complex automated workflows like PCR setup and serial dilutions.

Does the Opentrons integration support serial dilutions and plate replication?▼

Yes, the integration supports complex workflows such as serial dilutions, plate replication, and PCR setup. It provides detailed operational instructions and API references to execute these automated liquid handling tasks accurately.

What do I need to write liquid handling protocols for Flex and OT-2 robots?▼

You need to use the Opentrons Protocol API v2 with Python to develop protocols for Flex and OT-2 robots. The Skill provides scripts and API references to help you manage labware, pipettes, and hardware modules for your specific workflow.

Are there limitations when automating pipetting workflows with Opentrons?▼

While the API enables precise pipetting and module control, protocol complexity is bounded by the physical hardware constraints of Flex and OT-2 robots. Users must accurately define labware and module configurations in Python to avoid execution errors.

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