pacsomatic

Generate validated nf-core/pacsomatic samplesheets and launch scripts from tumor-normal BAM inputs.

74|5|Updated Dec 10, 2025
One-click install
npx skills add https://github.com/dralkh/seerai --skill pacsomatic-dralkh
Or copy as Structured Prompt for Agent
Please help me install this Agent Skill.
Skill: pacsomatic
Source: https://github.com/dralkh/seerai/tree/main/skills/pacsomatic
Command: npx skills add https://github.com/dralkh/seerai --skill pacsomatic-dralkh

SYSTEM DOCUMENTATION & REQUIREMENTS

💡 This Skill includes scripts (resource) and references (resource) components.

What problem does it solve?

pacsomatic removes the manual friction of preparing and launching matched tumor-normal somatic analysis runs, giving operators a validated path from BAM inputs to reproducible Nextflow execution.

Core Features & Use Cases

  • Input validation: Checks tumor and normal BAM paths, sample identifiers, reference mode, and available runtime prerequisites before launch.
  • Artifact generation: Builds a pacsomatic-compatible samplesheet, generated params YAML, and a reusable launch script.
  • Multi-backend execution: Supports local execution and scheduler submission for LSF, Slurm, PBS, and SGE with report-friendly run artifacts.
  • Troubleshooting support: Helps triage missing inputs, dependency gaps, and submission failures with clear next-step guidance.

Quick Start

Ask the pacsomatic skill to validate your matched tumor-normal BAM inputs, generate the samplesheet and launch script, and prepare a dry-run or scheduler submission with the reference mode you want.

Frequently Asked Questions about pacsomatic

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I prepare a samplesheet for nf-core pacsomatic tumor-normal analysis?

To validate matched tumor-normal BAM inputs for PacBio somatic analysis, provide patient and sample identifiers alongside tumor and normal BAM paths. The skill checks file paths, reference mode, and available runtime prerequisites before generating launch assets.

Can I run nf-core pacsomatic on Slurm or LSF clusters?

Yes, you can run nf-core pacsomatic on Slurm, LSF, PBS, or SGE clusters. The skill generates a reusable launch script configured for multi-backend execution, supporting both local execution and direct scheduler submission for PacBio HiFi somatic analysis.

What inputs are required to launch a PacBio HiFi somatic workflow?

Launching a PacBio HiFi somatic workflow requires patient and sample identifiers, tumor and normal BAM paths, and exactly one reference mode. The skill validates these inputs, checks indices, and confirms runtime dependencies before generating Nextflow artifacts.

Does Nextflow support dry-run validation for pacsomatic pipelines?

Nextflow supports dry-run validation for pacsomatic pipelines through this skill, which generates a params YAML and launch script to test the configuration. It verifies inputs, indices, and runtime dependencies without executing the full compute workload.

Why does my pacsomatic Nextflow submission fail on missing dependencies?

Your pacsomatic Nextflow submission may fail due to missing runtime dependencies or invalid indices. The skill provides troubleshooting support to triage dependency gaps, validate generated artifacts, and offer clear next-step guidance to resolve submission failures.