pathml

Process whole-slide images and construct spatial graphs for nucleus segmentation.

2|Updated Jun 4, 2026
One-click install
npx skills add https://github.com/Lord1Egypt/scientific-agent-toolkit --skill pathml-lord1egypt
Or copy as Structured Prompt for Agent
Please help me install this Agent Skill.
Skill: pathml
Source: https://github.com/Lord1Egypt/scientific-agent-toolkit/tree/main/scientific-skills/pathml
Command: npx skills add https://github.com/Lord1Egypt/scientific-agent-toolkit --skill pathml-lord1egypt

SYSTEM DOCUMENTATION & REQUIREMENTS

💡 This Skill requires pathml, torch, h5py, numpy, pandas, dask, scikit-image, and includes references (resource) components.

What problem does it solve?

This skill addresses the complexity of analyzing large-scale whole-slide pathology images by providing a unified, modular framework for image loading, preprocessing, and spatial analysis.

Core Features & Use Cases

  • Multi-Format WSI Support: Seamlessly load and process over 160 proprietary slide formats including SVS, NDPI, and DICOM.
  • Spatial & ML Workflows: Construct complex cell and tissue graphs, perform spatial proteomics analysis, and train deep learning models like HoVer-Net for nucleus segmentation.
  • Use Case: Researchers can use this skill to automate the analysis of multiplex immunofluorescence data from CODEX slides, from raw image loading to cell-type annotation and spatial neighborhood enrichment testing.

Quick Start

Use the pathml skill to load a whole-slide image and run a tissue detection and stain normalization pipeline on it.

Frequently Asked Questions about pathml

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I process whole-slide images for deep learning nucleus segmentation?

Load whole-slide images across over 160 formats including SVS and DICOM, apply stain normalization, and train deep learning models like HoVer-Net for nucleus segmentation. The framework uses HDF5 to manage large-scale data extraction and spatial graph construction efficiently.

What is the best way to run spatial analysis on multiplex immunofluorescence CODEX slides?

Spatial analysis on CODEX slides is handled by loading raw multiplex immunofluorescence images, performing cell-type annotation, and executing spatial neighborhood enrichment testing. This toolkit provides a unified workflow from image loading to spatial proteomics analysis.

Can I use PyTorch and HDF5 for scalable computational pathology workflows?

Yes, you can use PyTorch and HDF5 for scalable computational pathology workflows. The framework integrates PyTorch for deep learning-based feature extraction and HDF5 for managing large whole-slide image data, ensuring efficient spatial analysis and model training.

Does this computational pathology framework support proprietary slide formats like NDPI?

Yes, this computational pathology framework supports proprietary slide formats like NDPI. It seamlessly loads and processes over 160 proprietary whole-slide image formats, including SVS, NDPI, and DICOM, for diverse research workflows.

How do I build spatial cell and tissue graphs from histology images?

Build spatial cell and tissue graphs from histology images by loading whole-slide data and using the toolkit's spatial graph construction features. This enables complex spatial neighborhood enrichment testing and downstream spatial proteomics analysis.

When should I not use this toolkit for whole-slide image processing?

You should not use this toolkit if your whole-slide image processing workflow lacks the necessary dependencies or advanced computational resources. It requires PyTorch, HDF5, and Dask to handle large-scale spatial analysis and deep learning model training.