phylogenetics

Align biological sequences with MAFFT and infer evolutionary trees using IQ-TREE 2 or FastTree.

Updated Mar 10, 2026
One-click install
npx skills add https://github.com/Yezez9/Research-Agent --skill phylogenetics
Or copy as Structured Prompt for Agent
Please help me install this Agent Skill.
Skill: phylogenetics
Source: https://github.com/Yezez9/Research-Agent/tree/main/scientific-skills/phylogenetics
Command: npx skills add https://github.com/Yezez9/Research-Agent --skill phylogenetics

SYSTEM DOCUMENTATION & REQUIREMENTS

💡 This Skill includes scripts (resource) and references (resource) components.

What problem does it solve?

This Skill automates the complex process of reconstructing evolutionary relationships between biological sequences, enabling deep insights into evolution, disease spread, and molecular function.

Core Features & Use Cases

  • Sequence Alignment: Precisely aligns DNA, RNA, or protein sequences using MAFFT.
  • Phylogenetic Tree Inference: Builds evolutionary trees using robust methods like IQ-TREE 2 (Maximum Likelihood) or FastTree (fast approximation).
  • Visualization: Generates clear, interpretable tree visualizations.
  • Use Case: Trace the evolutionary path of a viral outbreak to understand its origins and spread, or analyze gene family evolution to discover functional relationships.

Quick Start

Run a full phylogenetic analysis on the provided DNA sequences in 'sequences.fasta'.

Frequently Asked Questions about phylogenetics

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I infer a phylogenetic tree from biological sequences?

To infer a phylogenetic tree, you align biological sequences using MAFFT and build evolutionary trees using IQ-TREE 2 or FastTree. This automates reconstructing evolutionary relationships to trace origins and analyze functional gene families.

What is the best way to align DNA and protein sequences for evolutionary analysis?

The best way to align DNA, RNA, or protein sequences for evolutionary analysis is using MAFFT. Precise sequence alignment is a required prerequisite before inferring accurate phylogenetic trees with maximum likelihood methods like IQ-TREE 2.

Do I need to install MAFFT and IQ-TREE 2 to run phylogenetic analysis?

Yes, you need to install MAFFT, IQ-TREE 2, and ETE3 to perform phylogenetic analysis. These external dependencies are required for executing sequence alignment, maximum likelihood tree building, and visualization respectively.

IQ-TREE 2 vs FastTree: which should I use for tree inference?

Use IQ-TREE 2 for robust Maximum Likelihood tree inference, or FastTree for a fast approximation. Both are supported for tree inference, letting you choose between computational precision and speed for your evolutionary analysis.

Can I trace a viral outbreak using molecular clock and phylogenetic analysis?

Yes, you can trace viral outbreak origins and spread using phylogenetic analysis. By aligning viral sequences with MAFFT and inferring evolutionary trees, you can facilitate molecular clock analyses and microbial genomics studies.

How do I visualize phylogenetic trees after inferring them?

You visualize phylogenetic trees using ETE3. After building your evolutionary tree with IQ-TREE 2 or FastTree, the visualization component generates clear, interpretable tree representations for evolutionary studies.