phylogenetics

Construct and analyze phylogenetic trees from biological sequences using MAFFT, IQ-TREE 2, and ETE3.

Updated Mar 10, 2026
One-click install
npx skills add https://github.com/felixboehm/biochem-allergy --skill phylogenetics-felixboehm
Or copy as Structured Prompt for Agent
Please help me install this Agent Skill.
Skill: phylogenetics
Source: https://github.com/felixboehm/biochem-allergy/tree/main/.claude/skills/phylogenetics
Command: npx skills add https://github.com/felixboehm/biochem-allergy --skill phylogenetics-felixboehm

SYSTEM DOCUMENTATION & REQUIREMENTS

💡 This Skill requires mafft, iqtree2, ete3, trimal, and includes scripts (resource) and references (resource) components.

What problem does it solve?

This Skill automates the complex process of reconstructing evolutionary relationships between biological sequences, enabling deep insights into genetic history and evolution.

Core Features & Use Cases

  • Sequence Alignment: Precisely aligns DNA, RNA, or protein sequences using MAFFT.
  • Phylogenetic Tree Inference: Builds evolutionary trees using Maximum Likelihood (IQ-TREE 2) or fast approximate methods (FastTree).
  • Tree Visualization: Renders clear, interpretable phylogenetic trees for analysis.
  • Use Case: Analyze the evolutionary spread of a virus by aligning its genome sequences, inferring a phylogenetic tree, and visualizing the relationships to understand transmission patterns.

Quick Start

Use the phylogenetics skill to build a phylogenetic tree from the provided aligned FASTA file 'sequences.fasta'.

Frequently Asked Questions about phylogenetics

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I build a phylogenetic tree from a FASTA file?

To build a phylogenetic tree from a FASTA file, this skill automates sequence alignment with MAFFT, infers the tree using IQ-TREE 2, and renders it via ETE3 for evolutionary analysis.

What is the best way to automate sequence alignment and tree inference for viral genomes?

Automating sequence alignment and tree inference for viral genomes is achieved by chaining MAFFT for precise alignment and IQ-TREE 2 for maximum likelihood tree construction to map evolutionary spread.

Can I use IQ-TREE 2 and MAFFT together for phylogenetic analysis?

Yes, IQ-TREE 2 and MAFFT are integrated together, aligning DNA, RNA, or protein sequences with MAFFT before inferring maximum likelihood phylogenetic trees using IQ-TREE 2.

Does this workflow support protein family analysis and microbial genomics?

Yes, the workflow supports protein family analysis and microbial genomics by aligning biological sequences and reconstructing evolutionary relationships to facilitate deep evolutionary studies.

How do I visualize a phylogenetic tree after inferring it?

To visualize a phylogenetic tree after inference, the skill uses ETE3 to render clear, interpretable tree structures directly from the generated maximum likelihood or fast approximate outputs.

When should I use FastTree instead of IQ-TREE 2 for tree inference?

You should use FastTree instead of IQ-TREE 2 when you need fast approximate phylogenetic tree inference methods, whereas IQ-TREE 2 provides maximum likelihood tree construction for precise evolutionary relationships.