pipeline-hic

Community

Process chromatin conformation data into contact maps and loops.

Authorammawla
Version1.0.0
Installs0

System Documentation

What problem does it solve?

This Skill enables researchers to convert raw Hi-C sequencing data into detailed contact matrices, identify topologically associating domains, and detect chromatin loops, facilitating the study of 3D genome structure.

Core Features & Use Cases

  • Hi-C Data Processing: From raw FASTQ files to multi-resolution contact maps in .hic and .mcool formats.
  • Loop and TAD Detection: Call chromatin loops with HiCCUPS and identify TAD boundaries for genome organization analysis.
  • Use Case: A researcher wants to analyze chromatin interactions in a specific cell line, generating contact matrices and loops for visualization and downstream functional annotation.

Quick Start

Load the raw Hi-C FASTQ files, specify reference genome and restriction enzyme, and run the Nextflow pipeline to generate contact matrices, loop calls, and quality metrics.

Dependency Matrix

Required Modules

bwapairtoolsjuicer_toolscooler

Components

scriptsreferences

💻 Claude Code Installation

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Please help me install this Skill:
Name: pipeline-hic
Download link: https://github.com/ammawla/encode-toolkit/archive/main.zip#pipeline-hic

Please download this .zip file, extract it, and install it in the .claude/skills/ directory.
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