pipeline-rnaseq

Community

Comprehensive RNA-seq processing from raw data to gene expression.

Authorammawla
Version1.0.0
Installs0

System Documentation

What problem does it solve?

This Skill streamlines the entire workflow of analyzing RNA sequencing data, generating accurate gene and transcript expression measures efficiently.

Core Features & Use Cases

  • End-to-End Workflow: Processes raw FASTQ files through quality control, alignment, quantification, and signal track generation.
  • Application Scope: Suitable for researchers and bioinformaticians conducting gene expression studies, transcript discovery, or preparing data for integrative analysis.
  • Functional Requirements: Ensures compatibility with ENCODE standards, handles stranded libraries, supports multiple computational environments, and provides QC metrics.

Quick Start

Run the RNA-seq analysis pipeline on your FASTQ files by specifying your input files, genome, and annotation, then execute the main script with the appropriate profile.

Dependency Matrix

Required Modules

STARRSEMKallistoFastQCTrim GaloresamtoolsbedGraphToBigWigRSeQCMultiQC

Components

scriptsreferencesassets

💻 Claude Code Installation

Recommended: Let Claude install automatically. Simply copy and paste the text below to Claude Code.

Please help me install this Skill:
Name: pipeline-rnaseq
Download link: https://github.com/ammawla/encode-toolkit/archive/main.zip#pipeline-rnaseq

Please download this .zip file, extract it, and install it in the .claude/skills/ directory.
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