pipeline-rnaseq
CommunityComprehensive RNA-seq processing from raw data to gene expression.
Education & Research#quality control#bioinformatics#alignment#nextflow#rna-seq#gene expression#quantification
Authorammawla
Version1.0.0
Installs0
System Documentation
What problem does it solve?
This Skill streamlines the entire workflow of analyzing RNA sequencing data, generating accurate gene and transcript expression measures efficiently.
Core Features & Use Cases
- End-to-End Workflow: Processes raw FASTQ files through quality control, alignment, quantification, and signal track generation.
- Application Scope: Suitable for researchers and bioinformaticians conducting gene expression studies, transcript discovery, or preparing data for integrative analysis.
- Functional Requirements: Ensures compatibility with ENCODE standards, handles stranded libraries, supports multiple computational environments, and provides QC metrics.
Quick Start
Run the RNA-seq analysis pipeline on your FASTQ files by specifying your input files, genome, and annotation, then execute the main script with the appropriate profile.
Dependency Matrix
Required Modules
STARRSEMKallistoFastQCTrim GaloresamtoolsbedGraphToBigWigRSeQCMultiQC
Components
scriptsreferencesassets
💻 Claude Code Installation
Recommended: Let Claude install automatically. Simply copy and paste the text below to Claude Code.
Please help me install this Skill: Name: pipeline-rnaseq Download link: https://github.com/ammawla/encode-toolkit/archive/main.zip#pipeline-rnaseq Please download this .zip file, extract it, and install it in the .claude/skills/ directory.
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