sarek

Plan and configure nf-core/sarek variant analysis runs from samplesheets.

1|Updated Jun 19, 2026
One-click install
npx skills add https://github.com/danilomonge/nf-claw --skill sarek
Or copy as Structured Prompt for Agent
Please help me install this Agent Skill.
Skill: sarek
Source: https://github.com/danilomonge/nf-claw/tree/main/pipelines/sarek
Command: npx skills add https://github.com/danilomonge/nf-claw --skill sarek

SYSTEM DOCUMENTATION & REQUIREMENTS

What problem does it solve?

nf-core/sarek automates germline and somatic variant analysis so researchers do not need to manually assemble complex Nextflow commands, parameter sets, and reference inputs for every sequencing project.

Core Features & Use Cases

  • End-to-end variant calling: Supports whole-genome, whole-exome, targeted, tumor-normal, and tumor-only workflows from raw reads through annotated outputs.
  • Strict parameter validation: Uses a pinned release schema to constrain inputs, required flags, allowed values, and file formats before the run starts.
  • Practical research use: Helps bioinformatics teams standardize sample sheet-driven analyses, reproduce prior runs, and choose the right tools for alignment, deduplication, calling, and annotation.

Quick Start

Ask for a complete nf-core/sarek run plan for my samplesheet, including the required parameters, recommended step, and the exact nfclaw command for my chosen sequencing scenario.

Frequently Asked Questions about sarek

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I configure an nf-core/sarek pipeline run for tumor-normal sequencing?

To configure a tumor-normal sequencing run, provide a validated samplesheet and required parameters to define the workflow. The Skill orchestrates execution planning by constraining inputs and flags against a pinned release schema.

What variant calling workflows can I plan using nf-core/sarek?

You can plan germline and somatic variant calling workflows for whole-genome, whole-exome, targeted, tumor-normal, and tumor-only sequencing runs. The Skill validates samplesheet inputs and required parameters for each scenario.

Can I use nf-core/sarek to validate my samplesheet inputs before execution?

Yes, nf-core/sarek validates samplesheet inputs, required parameters, allowed values, and file formats before the run starts. It relies on a pinned release schema to constrain inputs and ensure output contracts are met.

How do I get the exact Nextflow command for a whole-genome variant analysis run?

Request a complete run plan for your samplesheet to receive the exact Nextflow command. The Skill generates execution configurations including required parameters, recommended steps, and tool-specific run flags.

Does nf-core/sarek support targeted sequencing workflows alongside whole-exome analysis?

Yes, nf-core/sarek supports targeted, whole-exome, and whole-genome sequencing workflows. The Skill validates allowed values and run configurations for each sequencing scenario before execution.

Why does my nf-core/sarek run planning fail parameter validation?

Run planning fails parameter validation when inputs do not match the pinned release schema. The Skill strictly validates required flags, allowed values, and file formats before generating an execution plan.