scrnaseq

Run the pinned nf-core/scrnaseq pipeline with validated inputs and parameters.

1|Updated Jun 19, 2026
One-click install
npx skills add https://github.com/danilomonge/nf-claw --skill scrnaseq
Or copy as Structured Prompt for Agent
Please help me install this Agent Skill.
Skill: scrnaseq
Source: https://github.com/danilomonge/nf-claw/tree/main/pipelines/scrnaseq
Command: npx skills add https://github.com/danilomonge/nf-claw --skill scrnaseq

SYSTEM DOCUMENTATION & REQUIREMENTS

What problem does it solve?

This Skill removes the guesswork from running the nf-core/scrnaseq pipeline by turning a complex single-cell RNA-seq workflow into a validated, reproducible command sequence with the right inputs, parameters, and run targets.

Core Features & Use Cases

  • Pipeline Execution: Launch the pinned nf-core/scrnaseq release for single-cell RNA-seq data processing.
  • Input Validation: Use a strict samplesheet schema and required parameter checks to prevent malformed runs.
  • Release-Aware Runs: Select specific pipeline versions when you need behavior from a non-latest upstream release.
  • Provenance and Outputs: Capture run metadata, reports, and structured output directories for traceable analysis.
  • Use Case: A bioinformatics analyst can process 10x Genomics data, choose an aligner such as simpleaf or cellranger, and produce a complete results directory with MultiQC and execution provenance.

Quick Start

Use the scrnaseq skill to run your single-cell RNA-seq samplesheet through the pinned nf-core/scrnaseq pipeline and save the results to the output directory you specify.

Frequently Asked Questions about scrnaseq

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I run a single-cell RNA-seq pipeline using a validated samplesheet?

To run a single-cell RNA-seq pipeline, you launch the pinned nf-core/scrnaseq release by passing mandatory input and outdir arguments alongside your schema-checked samplesheet for validated, provenance-ready execution.

What aligners are supported for single-cell RNA-seq data processing?

Single-cell RNA-seq data processing supports aligners including simpleaf, cellranger, cellrangermulti, cellrangerarc, star, and kallisto across common sample sheet-driven 10x Genomics workflow scenarios.

Can I select a specific nf-core/scrnaseq version for reproducible analysis?

Yes, you can select specific nf-core/scrnaseq pipeline versions through release pinning, allowing you to lock behavior from a non-latest upstream release for reproducible analysis.

How does input validation prevent malformed single-cell RNA-seq runs?

Input validation prevents malformed runs by applying a strict samplesheet schema and checking mandatory parameters, ensuring only correctly formatted single-cell RNA-seq inputs proceed to execution.

What outputs and provenance data are generated from an scRNA-seq workflow?

An scRNA-seq workflow run produces a complete results directory containing MultiQC reports and execution provenance, capturing run metadata and structured outputs for traceable analysis.

Do I need Nextflow to execute the nf-core/scrnaseq workflow?

Executing the nf-core/scrnaseq workflow requires the Nextflow framework to run the pinned pipeline release and apply its schema-checked command-line flags for single-cell RNA-seq processing.