Analyze RNA velocity in single-cell RNA sequencing data with scVelo.
npx skills add https://github.com/Yezez9/Research-Agent --skill scvelo
Please help me install this Agent Skill. Skill: scvelo Source: https://github.com/Yezez9/Research-Agent/tree/main/scientific-skills/scvelo Command: npx skills add https://github.com/Yezez9/Research-Agent --skill scvelo
This Skill addresses the challenge of understanding dynamic biological processes like cell differentiation and development from static single-cell RNA sequencing data. It allows researchers to infer future cell states and identify key regulatory genes.
Run the RNA velocity analysis workflow on your AnnData object with spliced and unspliced layers.
High-intent search queries and answers about installing and using this skill.
RNA velocity is calculated by analyzing spliced and unspliced mRNA counts to estimate the rate of gene expression change. This Skill uses the scVelo package to compute these dynamics and infer future cell states from static scRNA-seq data.
Yes, this Skill integrates with Scanpy for preprocessing and visualization to infer cell differentiation trajectories. It leverages spliced and unspliced mRNA dynamics within your existing single-cell analysis workflow to determine trajectory directions.
Latent time estimation calculates a pseudotime that reflects a cell's progression through a dynamic biological process. This Skill computes latent time using scVelo to model gene expression dynamics and map out developmental timelines.
Driver genes are identified by analyzing spliced and unspliced mRNA dynamics to find genes most influential in cell fate decisions. This Skill uses scVelo to pinpoint key regulatory genes driving cell state transitions.
Yes, RNA velocity analysis requires an AnnData object containing spliced and unspliced mRNA layers. These layers are essential for scVelo to compute gene expression rates and infer trajectory directions accurately.
K-Dense-AIAnalyze single-cell RNA-seq splicing dynamics to infer cell state transitions.
felixboehmPerform RNA velocity analysis on single-cell RNA-seq data with scVelo.
CHENyiru3Automate single-cell RNA-seq analysis with Scanpy from QC to marker genes.
Estrella-231Infer RNA velocity and cell fate trajectories from single-cell RNA-seq AnnData.
