Infer RNA velocity from single-cell RNA-seq using spliced and unspliced mRNA kinetics.
npx skills add https://github.com/shushuzn/Rairos --skill scvelo-shushuzn
Please help me install this Agent Skill. Skill: scvelo Source: https://github.com/shushuzn/Rairos/tree/main/skills/scvelo Command: npx skills add https://github.com/shushuzn/Rairos --skill scvelo-shushuzn
scVelo helps you infer RNA velocity from snapshot single-cell RNA-seq data, revealing the direction of cell state transitions and estimating latent timing without requiring time-course experiments.
Use scvelo to compute RNA velocity and stream arrows on your existing UMAP by running the dynamical workflow on an AnnData object that contains layers["spliced"] and layers["unspliced"].
High-intent search queries and answers about installing and using this skill.
RNA velocity is inferred from snapshot single-cell RNA-seq data by modeling unspliced and spliced mRNA kinetics to estimate gene-wise state transition directions without requiring time-course experiments.
Computing trajectory direction and latent time requires an AnnData object containing spliced and unspliced layers, applying the dynamical model to reconstruct developmental trajectories and estimate shared latent time.
Yes, scvelo integrates with scanpy workflows by computing RNA velocity and projecting stream arrows onto your existing UMAP visualization using an AnnData object.
The stochastic model provides fast RNA velocity estimation, while the dynamical model is recommended for accurately computing latent time, trajectory directionality, and driver gene ranking.
Driver genes are identified by ranking genes whose kinetic dynamics best explain observed cell state transitions and quantifying model confidence for coherence across developmental or differentiation processes.
qinyan-aiInfer RNA velocity and latent time from spliced and unspliced mRNA in AnnData objects.
crazymsnIdentify cellular trajectories from scRNA-seq data using scVelo dynamical models.
JosephWoodallInfers cell state transitions from mRNA dynamics using scvelo and Scanpy.
Estrella-231Infer RNA velocity and cell fate trajectories from single-cell RNA-seq AnnData.
