Skills
.Work. You
Rest

starsolo-spliced-unspliced

Community

Fast RNA velocity with STARsolo Velocyto.

Data & Analytics#10x#scvelo#velocity-analysis#fastq#rna-velocity#starsolo#velocyto
Authortony-zhelonkin
Version1.0.0
Installs0

System Documentation

What problem does it solve?

Quantifies spliced, unspliced, and ambiguous counts from FASTQ inputs using STARsolo Velocyto mode to accelerate RNA velocity workflows and provide velocity-ready output without a separate velocyto.py step.

Core Features & Use Cases

  • One-pass STARsolo Velocyto mode maps reads and quantifies Spliced, Unspliced, and Ambiguous counts in a single run for efficient velocity analysis.
  • Produces Velocyto-formatted output compatible with downstream tools such as scVelo, CellRank, and VeloVI.
  • Generates output structures compatible with downstream pipelines and enables CellRanger-compatible velocity layers when needed.
  • Supports common 10X chemistries (V3, V2, 5') and BAM input modes as described in the workflow.

Quick Start

Run STARsolo with --soloFeatures Gene Velocyto on your FASTQ data to generate velocity-ready spliced, unspliced, and ambiguous matrices in a single pass.

Dependency Matrix

Required Modules

None required

Components

Standard package

💻 Claude Code Installation

Recommended: Let Claude install automatically. Simply copy and paste the text below to Claude Code.

Please help me install this Skill:
Name: starsolo-spliced-unspliced
Download link: https://github.com/tony-zhelonkin/SciAgent-toolkit/archive/main.zip#starsolo-spliced-unspliced

Please download this .zip file, extract it, and install it in the .claude/skills/ directory.
View Source Repository

Agent Skills Search Helper

Install a tiny helper to your Agent, search and equip skill from 471,000+ vetted skills library on demand.