trackpy-particle-tracking

To track particles in microscopy videos, this Skill uses the Crocker-Grier algorithm to locate particles with subpixel accuracy, link detections across frames into continuous trajectories, and filter short tracks for reliable motion analysis.

Mean squared displacement (MSD) analysis for single-particle tracking computes the average squared distance particles travel over time, allowing you to determine diffusion coefficients and distinguish between different types of motion in microscopy videos.

Yes, you can perform 3D particle tracking using trackpy, as this Skill supports both 2D and 3D tracking from various image formats loaded via pims to identify trajectories and calculate diffusion behavior.

Single-particle tracking in video microscopy supports various image formats loaded via pims, enabling you to process multi-frame image stacks like TIFF files for trajectory linking and MSD analysis.

To link particle detections across frames into continuous trajectories, this Skill applies the Crocker-Grier algorithm to connect identified particle locations in sequential frames and filters out short, incomplete tracks.

The best way to calculate diffusion coefficients from fluorescent molecule videos is to compute the ensemble mean squared displacement (MSD) of the linked particle trajectories, which quantifies their diffusion behavior over time.