alterlab-opentrons

Automate creation and execution of Opentrons Protocol API v2 protocols.

58|9|Updated Mar 16, 2026

Search Tags:#protocol #protocols #thermocycler #opentrons #pcr #api-v2 #labautomation

Ready to run

npx skills add https://github.com/AlterLab-IEU/AlterLab-Academic-Skills --skill alterlab-opentrons

💬 Prompt for ChatGPT / Claude Chat▼

Please help me install this Agent Skill.
Skill: alterlab-opentrons
Source: https://github.com/AlterLab-IEU/AlterLab-Academic-Skills/tree/main/skills/lab-integrations/alterlab-opentrons
Command: npx skills add https://github.com/AlterLab-IEU/AlterLab-Academic-Skills --skill alterlab-opentrons

SYSTEM DOCUMENTATION & REQUIREMENTS

💡 This Skill requires opentrons, and includes scripts (resource) and references (resource) components.

What problem does it solve?

Enables researchers to rapidly build, test, and execute Opentrons Protocol API v2 protocols for automated lab workflows.

Core Features & Use Cases

Protocol structure and metadata templates for OT-2 and Flex platforms
Comprehensive module control (temperature, magnetic, heater-shaker, thermocycler, and absorbance modules)
Labware loading, liquid handling operations, and multi-channel pipetting patterns
Reusable templates and references to accelerate protocol development and reproducibility
Use Case: quickly set up a standard PCR workflow or serial dilution with minimal boilerplate

Quick Start

Load an Opentrons protocol from scripts/ and run it on a Flex or OT-2 robot to begin automation.

Frequently Asked Questions about alterlab-opentrons

High-intent search queries and answers about installing and using this skill.

FAQPage Schema

How do I create Opentrons Protocol API v2 protocols for automated lab workflows?▼

To create Opentrons Protocol API v2 protocols, use this Skill's structured framework with metadata, module and labware loading helpers, and templates to accelerate Python-based protocol development for OT-2 and Flex robots.

Can I control a thermocycler and heater-shaker module using Opentrons API v2?▼

Yes, you can control a thermocycler, heater-shaker, temperature, magnetic, and absorbance modules using the comprehensive module control helpers provided for Opentrons API v2 protocol development.

What's the best way to set up a standard PCR workflow on an Opentrons Flex robot?▼

The best way to set up a standard PCR workflow on a Flex robot is loading a pre-built protocol from the provided scripts and templates, which minimizes boilerplate for rapid lab automation execution.

Does this Opentrons protocol integration support multi-channel pipetting patterns?▼

Yes, this Opentrons protocol integration supports multi-channel pipetting patterns alongside standard liquid handling operations and labware loading to automate common liquid-handling tasks efficiently.

Do I need the opentrons package to run these lab automation protocols?▼

Yes, you need the opentrons package installed as a dependency to load and execute these Python-based protocol API v2 scripts on your OT-2 or Flex robot for lab automation.

Are there reusable templates for serial dilution protocols in Opentrons API v2?▼

Yes, reusable templates and extensive references are provided to accelerate protocol development and reproducibility, allowing you to quickly set up serial dilution workflows with minimal boilerplate.