opentrons-integration

Automate Opentrons liquid handling with Python Protocol API v2 scripts.

557|98|Updated Nov 7, 2025
One-click install
npx skills add https://github.com/jimmc414/Kosmos --skill opentrons-integration-jimmc414
Or copy as Structured Prompt for Agent
Please help me install this Agent Skill.
Skill: opentrons-integration
Source: https://github.com/jimmc414/Kosmos/tree/main/kosmos-claude-scientific-skills/scientific-skills/opentrons-integration
Command: npx skills add https://github.com/jimmc414/Kosmos --skill opentrons-integration-jimmc414

SYSTEM DOCUMENTATION & REQUIREMENTS

💡 This Skill includes scripts (resource) and references (resource) components.

What problem does it solve?

This Skill automates complex liquid handling tasks on Opentrons robots, enabling precise and reproducible experimental workflows without manual intervention.

Core Features & Use Cases

  • Protocol Development: Write and execute Python-based protocols for Opentrons Flex and OT-2 robots.
  • Hardware Control: Manage pipettes, labware, and modules (temperature, magnetic, thermocycler, heater-shaker) for automated workflows.
  • Use Case: Automate the setup of 96-well plates for high-throughput screening, including serial dilutions, reagent additions, and sample transfers, ensuring consistency across experiments.

Quick Start

Use the opentrons-integration skill to write a Python protocol that transfers 100 microliters from source plate well A1 to destination plate well B1 using a p300 single pipette.

Frequently Asked Questions about opentrons-integration

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I automate liquid handling workflows on an Opentrons robot?

Automate liquid handling workflows on an Opentrons robot by writing and executing Python protocols using the Protocol API v2, managing pipettes, labware, and hardware modules to ensure reproducible scientific experiments.

Can I use Python to set up PCR and serial dilutions on Opentrons OT-2 or Flex robots?

Yes, you can use Python to set up PCR and serial dilutions on Opentrons OT-2 or Flex robots. The Protocol API v2 supports these advanced liquid handling features for high-throughput screening.

What is the Opentrons Protocol API v2 used for in lab automation?

The Opentrons Protocol API v2 is used in lab automation to create Python scripts for precise pipetting and hardware control on Opentrons robots, executing complex liquid handling tasks without manual intervention.

Does Opentrons protocol development support temperature, magnetic, and thermocycler modules?

Yes, Opentrons protocol development supports temperature, magnetic, thermocycler, and heater-shaker modules. The API v2 allows you to manage these hardware modules alongside pipettes and labware for automated workflows.

What is the best way to transfer specific volumes between labware using Opentrons?

The best way to transfer specific volumes between labware using Opentrons is to write a Python protocol defining the pipette, source well, and destination well, such as using a p300 single pipette to transfer 100 microliters from well A1 to well B1.

Why use automated liquid handling for high-throughput screening instead of manual pipetting?

Automated liquid handling ensures consistency and reproducibility across 96-well plate setups for high-throughput screening. Automating serial dilutions, reagent additions, and sample transfers eliminates manual intervention errors in experiments.