opentrons

Automate liquid handling using the Opentrons Python API v2 to write protocols that control OT-2 and Flex robots. The API lets you define deck configuration, load labware and modules, and script precise pipetting sequences—eliminating manual errors and ensuring reproducibility across serial dilutions, plate replication, and reagent distribution tasks.

Yes, the Opentrons Python API v2 supports hardware module integration including temperature control and magnetic separation. You script these operations alongside pipetting in a single protocol, enabling complex workflows like temperature-dependent reactions and magnetic bead-based separations on OT-2 and Flex robots.

Debug protocol execution by adjusting flow rates, validating deck configuration, and testing pipette control parameters in the Python script before running on hardware. The API provides data logging and error feedback to refine protocol robustness and ensure consistent performance across repeated runs.

You need Python knowledge and familiarity with your robot's hardware—OT-2 or Flex—and available labware, but the API is designed for researchers without deep automation experience. Starting with a minimal protocol skeleton that loads a 96-well plate and performs basic pipetting helps you learn the structure quickly.

Define deck configuration and labware in your Python protocol by specifying slot positions and module locations, then load labware definitions and hardware modules. The API handles spatial validation and ensures all components fit the robot's deck before execution.

Yes, the Opentrons Python API v2 integrates plate reading alongside liquid handling. You can script sequential operations—pipetting, incubation, and reading—in a single protocol to automate full assay workflows on OT-2 and Flex robots.