bio-gene-calling

Call genes in microbial genomes and generate GFF3, FAA, and FNA outputs.

7|1|Updated Feb 2, 2026
One-click install
npx skills add https://github.com/fmschulz/omics-skills --skill bio-gene-calling
Or copy as Structured Prompt for Agent
Please help me install this Agent Skill.
Skill: bio-gene-calling
Source: https://github.com/fmschulz/omics-skills/tree/main/skills/bio-gene-calling
Command: npx skills add https://github.com/fmschulz/omics-skills --skill bio-gene-calling

SYSTEM DOCUMENTATION & REQUIREMENTS

What problem does it solve?

Call genes and annotate basic features for prokaryotes, viruses, and eukaryotes.

Core Features & Use Cases

  • Gene calling across diverse organisms: select appropriate caller (Prodigal, Prodigal-GV, BRAKER, AUGUSTUS) based on organism class and data availability.
  • Output generation: produce standard formats like GFF3, FAA, and FNA; optionally detect tRNAs and rRNAs.
  • Workflow integration: applicable to contigs or bins in metagenomic or isolated genomes; suitable for end-to-end annotation pipelines.

Quick Start

Run bio-gene-calling on input contigs to produce GFF3, FAA, and FNA outputs.

Frequently Asked Questions about bio-gene-calling

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I predict gene features in prokaryotic and viral genomes?

To predict gene features in prokaryotic and viral genomes, you can use this skill to invoke Prodigal or Prodigal-GV for gene calling and generate standardized GFF3, FAA, and FNA outputs. It automatically selects the appropriate caller based on organism class and data availability.

Can I detect tRNA and rRNA sequences during genome annotation?

Yes, you can detect tRNA and rRNA sequences during genome annotation. The skill performs basic feature annotation and offers optional tRNA and rRNA detection alongside the primary gene calling workflow.

How do I annotate gene features in metagenomic bins or contigs?

You can annotate gene features in metagenomic bins or contigs by running this skill, which integrates into end-to-end annotation pipelines and supports reproducible workflows for both isolated and metagenomic genomes.

What output formats are generated by microbial gene calling workflows?

Microbial gene calling workflows generate standardized GFF3, FAA, and FNA output formats. The skill validates results to ensure non-empty sequences and properly formatted annotations for downstream analysis.

Does this gene caller support eukaryotic genome annotation?

Yes, it supports eukaryotic genome annotation by invoking BRAKER or AUGUSTUS as the gene caller. The skill covers prokaryotes, viruses, and eukaryotes across contigs or bins.

What are the limitations of using automated gene callers for microbial genomes?

Automated gene callers require proper input validation and enforce checks to ensure non-empty sequences. The skill validates results to ensure properly formatted annotations, but accuracy depends on selecting the correct caller for the organism class.