bioinformatics-init-analysis

Automate single-cell data analysis from loading to HTML report generation.

708|51|Updated Mar 10, 2026
One-click install
npx skills add https://github.com/LigphiDonk/Oh-my--paper --skill bioinformatics-init-analysis-ligphidonk
Or copy as Structured Prompt for Agent
Please help me install this Agent Skill.
Skill: bioinformatics-init-analysis
Source: https://github.com/LigphiDonk/Oh-my--paper/tree/main/src-tauri/resources/skills/bioinformatics-init-analysis
Command: npx skills add https://github.com/LigphiDonk/Oh-my--paper --skill bioinformatics-init-analysis-ligphidonk

SYSTEM DOCUMENTATION & REQUIREMENTS

💡 This Skill requires numpy, pandas, scanpy, anndata, matplotlib, seaborn, scikit-learn, fcsparser, and includes scripts (resource) and references (resource) components.

What problem does it solve?

This tool automates the end-to-end analysis of high-dimensional single-cell biology data, transforming raw measurements into ready-to-interpret results and a shareable report.

Core Features & Use Cases

  • Automated 7-step workflow: Load data, QC, normalize, reduce dimensionality, cluster, analyze markers, and generate a clinical/technical report.
  • Data-type awareness: Auto-detects CyTOF, scRNA-seq, and flow cytometry formats and adapts steps accordingly.
  • Exploratory and reporting: Produces both visuals (UMAP, PCA, heatmaps) and a self-contained HTML report with a machine-readable summary.

Quick Start

Run the 7-step pipeline on your dataset to obtain a processed AnnData object and a comprehensive report.

Frequently Asked Questions about bioinformatics-init-analysis

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I automate single-cell RNA-seq analysis from raw data to report?

You can automate single-cell RNA-seq analysis by running a 7-step pipeline that handles data loading, QC, normalization, dimensionality reduction, clustering, and marker analysis to output a processed AnnData object and HTML report.

What is the best way to run clustering and normalization for CyTOF data?

The best way to run clustering and normalization for CyTOF data is using an automated pipeline that auto-detects the format and applies modular scripts to generate UMAP visuals and a structured JSON summary.

Does this pipeline support flow cytometry datasets?

Yes, the pipeline supports flow cytometry datasets by auto-detecting the data type and adapting the analysis steps accordingly to produce dimensionality reduction visuals and a self-contained HTML report.

Can I use scanpy and anndata for single-cell clustering and visualization?

Yes, you can use scanpy and anndata for single-cell clustering and visualization, as the pipeline leverages these dependencies to process high-dimensional data and generate UMAP, PCA, and heatmap visuals.

How does automated dimensionality reduction work for high-dimensional single-cell biology data?

Automated dimensionality reduction for high-dimensional single-cell biology data works by processing raw measurements through a modular workflow that outputs ready-to-interpret visuals like PCA and UMAP plots.

Why do I need to generate a clinical report from scRNA-seq data?

You need to generate a clinical report from scRNA-seq data to transform raw measurements into ready-to-interpret results, providing a shareable HTML report with a machine-readable JSON summary for downstream evaluation.