celltypeannotation

Annotate scRNA-seq clustering results with cell type labels using multiple tools.

22|4|Updated May 18, 2021
One-click install
npx skills add https://github.com/pwwang/immunopipe --skill celltypeannotation
Or copy as Structured Prompt for Agent
Please help me install this Agent Skill.
Skill: celltypeannotation
Source: https://github.com/pwwang/immunopipe/tree/main/skills/celltypeannotation
Command: npx skills add https://github.com/pwwang/immunopipe --skill celltypeannotation

SYSTEM DOCUMENTATION & REQUIREMENTS

What problem does it solve?

Annotates clustering results with biological cell type labels to give meaningful identities to clusters, enabling downstream interpretation and comparison across samples.

Core Features & Use Cases

  • Direct assignment
  • ScType
  • scCATCH
  • hitype
  • CellTypist
  • Seamless integration with Seurat objects and h5ad inputs for PBMC and multi-sample datasets

Quick Start

Supply your Seurat clustering results and run this workflow to obtain labeled cell types for each cluster.

Frequently Asked Questions about celltypeannotation

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I annotate scRNA-seq clustering results with biological cell types?

Cell-type annotation for scRNA-seq data is performed by applying automated tools like ScType, scCATCH, hitype, or CellTypist to assign biological labels to clusters for downstream interpretation.

Can I use CellTypist or scCATCH to label immune cell clusters in a Seurat object?

Yes, you can use CellTypist and scCATCH for immune cell datasets. The workflow seamlessly integrates with Seurat objects to deliver labeled cell types for each cluster.

Does this cell-type annotation workflow support h5ad files and multi-sample studies?

Yes, the workflow supports h5ad inputs and applies to post-clustering analyses across multi-sample datasets, enabling configurable input formats and output columns for reproducible annotation.

What is the best way to compare direct assignment vs automated annotation tools for scRNA-seq data?

You can compare direct assignment against automated tools like ScType, scCATCH, hitype, or CellTypist by configuring tool choices and tissue specifications within the same reproducible annotation workflow.

How do I configure tissue specifications and database paths for scCATCH or ScType?

You can configure tissue specifications, database paths, tool choices, and output columns within the workflow to ensure annotation tools correctly match your specific scRNA-seq dataset context.