design-base-editor-sgrnas

To design base editor sgRNAs, provide a gene symbol, organism, and editor type. The tool fetches exonic sequences from Ensembl, scans for NGG PAM sites, filters guides with editable bases, and returns a ranked guide table with bystander reports.

CBE sgRNA design targets C bases for cytosine base editing, while ABE sgRNA design targets A bases for adenine base editing. The tool filters guides whose editing window contains the appropriate base and scores them for efficiency and bystander risk.

Yes, you can design base editing sgRNAs for human, mouse, and monkey genomes. Specify the organism input alongside the gene symbol and editor type to fetch the correct exonic sequences from Ensembl for PAM-aware scanning.

The tool evaluates bystander edits by scanning the editing window of each guide for additional C or A bases near the target. It generates a bystander report and editing heatmaps to help you minimize unwanted off-target nucleotide changes.

You need a gene symbol, organism, editor type, and editing window parameters. The tool uses these inputs to fetch exonic sequences, scan PAM sites on both strands, and output ranked guide tables, top guides, and plotting figures.

Yes, the tool supports dual-base editors alongside CBE and ABE. It scans both strands for PAM sites, filters guides where the editing window contains C or A bases, and ranks them for both single and dual-base editing applications.