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design_sgrnas_by_gene

Retrieve ranked CRISPR sgRNA candidates from GuidePro for human, monkey, and mouse genes.

64|12|Updated Apr 7, 2026
Search Tags:#design#reference#crispr#genelist#sgrna#guidepro
One-click install
npx skills add https://github.com/MDhewei/bioinfor-claw --skill design-sgrnas-by-gene
Or copy as Structured Prompt for Agent▼
Please help me install this Agent Skill.
Skill: design_sgrnas_by_gene
Source: https://github.com/MDhewei/bioinfor-claw/tree/main/crispr-design-and-analysis/design-sgrnas-by-gene
Command: npx skills add https://github.com/MDhewei/bioinfor-claw --skill design-sgrnas-by-gene

SYSTEM DOCUMENTATION & REQUIREMENTS

💡 This Skill requires pandas>=2.0.0, and includes scripts (resource) components.

What problem does it solve?

Retrieve CRISPR sgRNA candidates for a gene or gene list from the GuidePro genome-wide reference table, filtering and ranking results to support knockout design workflows across human, monkey, and mouse.

Core Features & Use Cases

  • Retrieve sgRNA candidates for one gene, multiple genes, or a gene list from GuidePro.
  • Automatically detect gene columns, handle gene lists from files, and output a ranked TSV with top guides per gene.
  • Cache GuidePro reference locally, support --top-n-per-gene, optional deduplication, and optional summary reporting.

Quick Start

Run the skill to fetch GuidePro sgRNA candidates for a gene or gene list and output the top guides per gene

Frequently Asked Questions about design_sgrnas_by_gene

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I design CRISPR sgRNA candidates for a list of genes?▼

CRISPR sgRNA candidates are retrieved from the GuidePro genome-wide reference table, which filters and ranks guides across human, monkey, and mouse genomes to support knockout design workflows.

How do I retrieve top-N sgRNA guides per gene from GuidePro?▼

Retrieve top-N sgRNA guides per gene by running the skill with the --top-n-per-gene option, which filters the GuidePro reference table and outputs a ranked TSV file for review or ordering.

Can I use GuidePro sgRNA candidates for mouse and monkey genomes?▼

Yes, GuidePro sgRNA candidates support human, monkey, and mouse genomes, allowing you to specify the target genome when retrieving ranked guides for your knockout design workflows.

What's the best way to batch process a gene list file for CRISPR sgRNA selection?▼

Batch processing a gene list file involves passing it to the skill, which automatically detects gene columns, handles deduplication, and outputs a ranked TSV with selected sgRNA candidates from GuidePro.

Does the GuidePro sgRNA retrieval tool require internet access every time?▼

No, the GuidePro sgRNA retrieval tool implements local caching of the reference table, meaning repeated queries for sgRNA candidates use the cached data without requiring continuous internet access.

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