crispr-library-design

To design a pooled CRISPR sgRNA library, input a comma-separated list of gene symbols along with desired guides per gene, controls, and PAM constraints. The tool selects top guides, generates non-targeting controls, and outputs ready-to-synthesize oligo sequences in TSV, CSV, or FASTA formats.

Generating oligo synthesis orders for CRISPR screens involves applying efficiency and off-target scoring to rank sgRNA guides per gene. This skill outputs vector-ready sequences with optional flanking regions, producing TSV, CSV, and FASTA files plus summary plots for direct synthesis.

CRISPR library design supports both human and mouse genomes for target gene screening. You can input gene symbols from either species, apply PAM constraints and GC filters, and generate enrichment libraries with non-targeting controls and vector-ready outputs.

Yes, you can include non-targeting controls in your sgRNA library design by specifying the number of controls needed in the input parameters. The tool automatically generates these controls alongside top-ranked guides for your target genes.

sgRNA guides are ranked and selected by applying efficiency scoring and off-target analysis to ensure library quality. The process uses PAM constraints and GC filters to identify top guides per gene for your pooled CRISPR library.

Available output formats for synthesized CRISPR oligo sequences include TSV, CSV, and FASTA. The design process also generates summary statistics and plots to visualize library quality alongside the vector-ready sequence files.