crispr-library-design
CommunityDesign CRISPR sgRNA libraries with top guides.
Data & Analytics#ensembl#library-design#crispr#off-target#sgrna-design#gene-targeting#oligo-synthesis
AuthorMDhewei
Version1.0.0
Installs0
System Documentation
What problem does it solve?
This skill automates the design of pooled CRISPR sgRNA libraries, selecting top guides per gene and generating oligo orders for synthesis, streamlining library creation for large-scale screens.
Core Features & Use Cases
- Design sgRNA guides for each target gene using PAM constraints and GC filters, with per-gene ranking.
- Apply efficiency and off-target scoring to identify top guides and ensure library quality.
- Generate non-targeting controls and output ready-to-synthesize oligo sequences, plus summary statistics and plots.
- Supports vector design with optional flanking sequences and multiple output formats (TSV/CSV/FASTA).
Quick Start
Run the design script with a comma-separated list of gene symbols to produce a ready-to-synthesize CRISPR sgRNA library for your targets.
Dependency Matrix
Required Modules
requestsnumpypandasmatplotlib
Components
scripts
💻 Claude Code Installation
Recommended: Let Claude install automatically. Simply copy and paste the text below to Claude Code.
Please help me install this Skill: Name: crispr-library-design Download link: https://github.com/MDhewei/bioinfor-claw/archive/main.zip#crispr-library-design Please download this .zip file, extract it, and install it in the .claude/skills/ directory.
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