Design primers for Golden Gate and multi-fragment DNA assembly workflows.
npx skills add https://github.com/Zurybr/lefarma-skills --skill dna-assembly
Please help me install this Agent Skill. Skill: dna-assembly Source: https://github.com/Zurybr/lefarma-skills/tree/main/letta/benchmarks/trajectory-only/dna-assembly Command: npx skills add https://github.com/Zurybr/lefarma-skills --skill dna-assembly
This Skill streamlines the complex process of designing primers for Golden Gate assembly and other multi-fragment DNA assembly workflows, reducing errors and increasing success rates.
Use the dna-assembly skill to design primers for a 4-fragment linear Golden Gate assembly.
High-intent search queries and answers about installing and using this skill.
Golden Gate assembly primer design requires selecting correct Type IIS restriction enzyme overhangs and structuring primer orientation for scarless multi-fragment ligation. This Skill provides step-by-step guidance for overhang selection, primer structure, and assembly simulation to ensure accurate cloning outcomes.
In-silico multi-fragment assembly simulation verifies the final DNA construct and confirms scarless ligation before wet lab execution. This Skill emphasizes simulating assembly outcomes to check overhang compatibility and ensure the correct product is formed.
Choosing Type IIS cloning overhangs involves selecting unique sequences that define fragment order and ensure scarless assembly. This Skill details overhang requirements and primer orientation to guide you through selecting compatible overhangs for multi-fragment DNA assembly.
Yes, this Skill supports multi-fragment DNA assembly workflows, guiding you through assembling multiple fragments into a plasmid using Golden Gate cloning. It covers designing overhangs, checking internal restriction sites, and verifying the final assembly product.
Golden Gate assembly fails when primers have incorrect overhangs, internal Type IIS restriction sites, or improper orientation. This Skill provides a verification checklist to ensure all design parameters are met and simulates assembly to prevent failed cloning outcomes.
IDEA-XLAnalyze DNA, RNA, and protein sequences for length, GC content, ORFs, and translations.
wuyoscarAutomate DNACauldron simulations to validate multi-fragment Golden Gate DNA assembly.
Runchuan-BUAnalyzes nucleotide and protein sequences for GC content, ORFs, restriction sites, and primers.
farnunglabDesign primers for LIC cloning, Gibson/HiFi assembly, and sequencing from NCBI accessions or raw sequences.
