skills.rest
Runchuan-BU
Runchuan-BUCOMMUNITY
Explore all skills by this author →
GitHub

sequence-analysis

Analyzes nucleotide and protein sequences for GC content, ORFs, restriction sites, and primers.

401|53|Updated Feb 26, 2026
Search Tags:#translation#biopython#sequence-analysis#orf#primer-design#gc-content#restriction-sites
One-click install
npx skills add https://github.com/Runchuan-BU/BioClaw --skill sequence-analysis
Or copy as Structured Prompt for Agent▼
Please help me install this Agent Skill.
Skill: sequence-analysis
Source: https://github.com/Runchuan-BU/BioClaw/tree/main/.claude/skills/sequence-analysis
Command: npx skills add https://github.com/Runchuan-BU/BioClaw --skill sequence-analysis

SYSTEM DOCUMENTATION & REQUIREMENTS

What problem does it solve?

Analyze raw nucleotide or protein sequences quickly to extract key properties, translate coding regions, identify ORFs, map restriction sites, and produce primer suggestions so researchers avoid manual, error-prone sequence inspection.

Core Features & Use Cases

  • Sequence properties: length, GC content, composition, complement and reverse complement.
  • Translation & ORF finding: translate coding frames and locate open reading frames on both strands.
  • Restriction mapping & primer design: detect common enzyme cut sites and propose basic forward/reverse primers with melting temperature estimates.
  • Alignment support: perform simple pairwise or basic multiple-sequence alignment when multiple sequences are provided.
  • Use case: paste a plasmid or gene sequence to get GC%, translate the coding region, list ORFs above a size threshold, and check for EcoRI/BamHI/HindIII sites before cloning.

Quick Start

Paste the sequence text or FASTA into chat and ask: please analyze this sequence and report length, GC content, translations, ORFs, restriction sites, and propose basic primers.

Frequently Asked Questions about sequence-analysis

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I calculate GC content and translate a DNA sequence?▼

Calculate GC content and translate DNA by pasting raw FASTA text to compute length, composition, and translate coding frames into protein sequences directly.

What is the best way to find open reading frames in a nucleotide sequence?▼

Find open reading frames by scanning both strands of a nucleotide sequence to locate coding regions above a specified size threshold for quick identification.

How do I map restriction enzyme sites like EcoRI and BamHI for cloning?▼

Map restriction enzyme sites like EcoRI and BamHI by inputting a plasmid sequence to detect common cut sites and generate basic forward and reverse primer suggestions.

Can I perform multiple sequence alignment using BioPython-style parsing?▼

Perform simple pairwise or basic multiple sequence alignment using BioPython-style sequence parsing and command-line alignment fallbacks when multiple sequences are provided.

Does sequence analysis provide melting temperature estimates for primer design?▼

Sequence analysis provides melting temperature estimates for primer design by proposing basic forward and reverse primers alongside restriction site mapping outputs.

Related Skills

Explore More Education & Research Skills→
omar-A-hassanomar-A-hassan
18

biopython

Parse FASTA files and analyze DNA, RNA, and protein sequences.

Community
Intermediate
exon-researchexon-research
475

sequence

Translate DNA sequences, detect ORFs, and check primers locally.

Official
Intermediate
jaechang-hitsjaechang-hits
298

biopython-sequence-analysis

Perform sequence analysis with Biopython, including NCBI Entrez downloads and BLAST searches.

Community
Advanced
yf8578yf8578
1

sequence-analysis

Analyze DNA, RNA, and protein sequences with BioPython and command-line tools.

Community
Intermediate