skills.rest
yf8578
yf8578COMMUNITY
Explore all skills by this author →
GitHub

sequence-analysis

Analyze DNA, RNA, and protein sequences with BioPython and command-line tools.

1|Updated Mar 12, 2026
Search Tags:#bioinformatics#sequence analysis#protein#dna#orf#rna#primer design
One-click install
npx skills add https://github.com/yf8578/clawomics --skill sequence-analysis-yf8578
Or copy as Structured Prompt for Agent▼
Please help me install this Agent Skill.
Skill: sequence-analysis
Source: https://github.com/yf8578/clawomics/tree/main/skills/sequence-analysis
Command: npx skills add https://github.com/yf8578/clawomics --skill sequence-analysis-yf8578

SYSTEM DOCUMENTATION & REQUIREMENTS

💡 This Skill requires biopython, and includes scripts (resource) and references (resource) components.

What problem does it solve?

This Skill provides comprehensive analysis for DNA, RNA, and protein sequences, enabling users to understand sequence properties, identify key features, and perform various biological computations.

Core Features & Use Cases

  • Sequence Properties: Calculate length, GC content, and generate complements.
  • Translation: Translate DNA/RNA sequences into protein sequences.
  • ORF Finding: Identify Open Reading Frames within a sequence.
  • Restriction Site Analysis: Detect cutting sites for specified restriction enzymes.
  • Primer Design: Basic primer design with melting temperature calculation.
  • Multiple Sequence Alignment: Perform pairwise or multiple sequence alignments.
  • Use Case: A researcher has a newly sequenced gene and wants to find potential protein-coding regions, check its GC content, and identify restriction enzyme sites for cloning.

Quick Start

Analyze the provided DNA sequence for its length, GC content, and potential ORFs.

Frequently Asked Questions about sequence-analysis

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I calculate GC content and find open reading frames in a DNA sequence?▼

To analyze DNA sequences, you can calculate GC content and identify open reading frames (ORFs) by inputting your raw sequence data. The tool processes the sequence to return length, GC percentage, and potential protein-coding regions.

Can I translate DNA or RNA sequences into protein sequences?▼

Yes, you can translate DNA or RNA sequences into protein sequences. The tool processes the nucleotide input to generate the corresponding amino acid sequence, helping you understand potential protein products from your genetic data.

Do I need BioPython to perform restriction site analysis and primer design?▼

Yes, BioPython is required for core sequence manipulation including restriction site analysis and primer design. Optional command-line tools are also supported for performing advanced multiple sequence alignments.

What is the best way to design PCR primers and calculate melting temperature?▼

The best way to design PCR primers and calculate melting temperature is to input your target DNA sequence. The tool generates basic primer sequences and provides melting temperature calculations to ensure optimal PCR conditions.

Does this tool support multiple sequence alignment for newly sequenced genes?▼

Yes, multiple sequence alignment is supported for newly sequenced genes. The tool performs pairwise or multiple sequence alignments using BioPython and optional command-line tools to help compare genetic sequences.

Related Skills

Explore More Education & Research Skills→
fuzzy-dynamicsfuzzy-dynamics
1

scikit-bio

Perform end-to-end biological data analyses in Python with scikit-bio.

Official
Advanced
pradyumnasagarpradyumnasagar
6

sequence-properties

Compute GC content, molecular weight, and melting temperature for DNA, RNA, and protein sequences.

Community
Intermediate
Runchuan-BURunchuan-BU
401

sequence-analysis

Analyzes nucleotide and protein sequences for GC content, ORFs, restriction sites, and primers.

Community
Intermediate
jonnabiojonnabio
6

biopython

Automate molecular biology tasks using Biopython for sequence analysis and BLAST operations.

Community
Advanced