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lifesciences-crispr

Validate CRISPR synthetic lethality claims using BioGRID ORCS screens.

Updated Feb 5, 2026
Search Tags:#crispr#synthetic lethality#orcs#biogrid#essentiality#cell lines
One-click install
npx skills add https://github.com/donbr/lifesciences-deepagents --skill lifesciences-crispr
Or copy as Structured Prompt for Agent▼
Please help me install this Agent Skill.
Skill: lifesciences-crispr
Source: https://github.com/donbr/lifesciences-deepagents/tree/main/.claude/skills/lifesciences-crispr
Command: npx skills add https://github.com/donbr/lifesciences-deepagents --skill lifesciences-crispr

SYSTEM DOCUMENTATION & REQUIREMENTS

💡 This Skill includes references (resource) components.

What problem does it solve?

Validates synthetic lethality claims from CRISPR knockout screens using BioGRID ORCS 5-phase workflow. This skill should be used when the user asks to "validate synthetic lethality", "query CRISPR essentiality data", "find gene dependencies", "compare cell line screens", or mentions BioGRID ORCS, gene knockout data, essentiality scores (CERES, MAGeCK, BAGEL), or asks to validate claims from published CRISPR papers.

Core Features & Use Cases

  • 5-phase workflow for CRISPR essentiality validation using BioGRID ORCS.
  • Resolve gene identifiers, fetch essential screens, annotate cells, and compare dependencies with CERES, MAGeCK, and BAGEL scores.
  • Generate evidence-backed conclusions with provenance across diverse cell lines and genetic backgrounds.

Quick Start

Query CRISPR essentiality screens for a gene in ORCS and interpret significant synthetic-lethality scores across cell lines.

Frequently Asked Questions about lifesciences-crispr

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I validate CRISPR synthetic lethality claims using BioGRID ORCS screens?▼

Validate CRISPR synthetic lethality by resolving gene identifiers, fetching BioGRID ORCS essentiality screens, annotating cell lines, and comparing CERES, MAGeCK, and BAGEL dependency scores across contexts.

What essentiality scores can I compare across cell lines for CRISPR knockout data?▼

Compare CERES, MAGeCK, and BAGEL essentiality scores across diverse cell lines and genetic backgrounds by querying BioGRID ORCS endpoints and filtering results with the hit=yes parameter.

How do I query BioGRID ORCS to find gene dependencies from CRISPR knockout screens?▼

Query BioGRID ORCS CRISPR screens by resolving target gene identifiers, collecting essentiality data via orcsws.thebiogrid.org endpoints using an API key, and scoring dependencies across multiple cell lines.

Does the BioGRID ORCS CRISPR validation workflow require an API key?▼

Yes, validating CRISPR synthetic lethality with BioGRID ORCS requires an API key to access orcsws.thebiogrid.org endpoints, filter screens with hit=yes, and retrieve essentiality scores across cell lines.

What happens when BioGRID ORCS has no CRISPR essentiality data for a gene?▼

When BioGRID ORCS CRISPR screens lack essentiality data for a queried gene, the validation workflow returns no results rather than inferring dependency scores, ensuring evidence-backed conclusions with provenance.

Can I validate synthetic lethality from published CRISPR papers across different genetic backgrounds?▼

Validate synthetic lethality claims from published CRISPR papers by fetching BioGRID ORCS screens, annotating cell lines, and comparing CERES, MAGeCK, and BAGEL scores across diverse genetic backgrounds.

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