opentrons-integration

Writes Opentrons Protocol API v2 scripts for Flex and OT-2 automation.

74|5|Updated Dec 10, 2025
One-click install
npx skills add https://github.com/dralkh/seerai --skill opentrons-integration-dralkh
Or copy as Structured Prompt for Agent
Please help me install this Agent Skill.
Skill: opentrons-integration
Source: https://github.com/dralkh/seerai/tree/main/skills/opentrons-integration
Command: npx skills add https://github.com/dralkh/seerai --skill opentrons-integration-dralkh

SYSTEM DOCUMENTATION & REQUIREMENTS

💡 This Skill requires opentrons, and includes scripts (resource) and references (resource) components.

What problem does it solve?

This Skill removes the complexity of writing and maintaining Opentrons robot protocols by giving you ready-to-use guidance for pipetting workflows, deck setup, and module control.

Core Features & Use Cases

  • Protocol authoring: Create valid Opentrons Protocol API v2 scripts with metadata, robot type settings, and a standard run function.
  • Lab automation workflows: Configure labware, tip racks, pipettes, and modules for tasks such as serial dilution, plate replication, and PCR setup.
  • Hardware control: Use temperature, magnetic, heater-shaker, and thermocycler modules with correct sequencing and handling.
  • Use Case: A researcher needs a reproducible PCR workflow for a Flex robot, including reagent distribution, sample addition, and thermal cycling with the thermocycler module.

Quick Start

Ask the assistant to generate a Flex-compatible Opentrons PCR protocol with explicit deck positions, tip racks, reagent loading, and thermocycler steps.

Frequently Asked Questions about opentrons-integration

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I write an Opentrons protocol for PCR setup on a Flex robot?

Generate a Flex-compatible Opentrons Protocol API v2 script by defining deck positions, tip racks, reagent loading, and thermocycler steps. This ensures accurate liquid tracking and safe execution of pipetting and thermal cycling operations.

How do I configure labware and modules for Opentrons liquid handling automation?

Configure Opentrons liquid handling by assigning labware, tip racks, and pipettes to deck positions, then initialize temperature, magnetic, heater-shaker, or thermocycler modules with correct sequencing for safe workflow execution.

Can I use the Opentrons API for serial dilution workflows on the OT-2 robot?

Yes, the Opentrons API supports serial dilution workflows on the OT-2 robot by utilizing Protocol API v2 scripts to accurately track liquids and sequence pipette operations across configured labware and tip racks.

What is needed to simulate Opentrons protocols before running on hardware?

Simulating Opentrons protocols requires a valid Protocol API v2 script with accurate deck and tip-rack configuration, liquid tracking, and properly sequenced pipette operations to verify safe execution logic before running on hardware.

Does Opentrons Protocol API v2 support thermocycler and heater-shaker modules?

Yes, Opentrons Protocol API v2 supports thermocycler and heater-shaker modules, enabling correct sequencing and handling for complex lab automation workflows. This ensures safe execution of temperature control and mixing operations.