opentrons-integration

To write an Opentrons Protocol API v2 script for PCR setup, define protocol metadata and a run(context) entry point, then load pipettes, labware, and thermocycler modules to execute liquid handling transfers and temperature cycling.

Yes, you can control a heater-shaker and thermocycler in the same Opentrons protocol by loading both hardware modules into your deck configuration and specifying their commands within the run() function.

The best way to automate serial dilution on an OT-2 robot is to use Protocol API v2 to program transfer and mixing steps across a 96-well plate with efficient tip management strategies.

You configure labware and pipettes for Opentrons Flex simulations by using Protocol API v2 to load instruments onto the deck and adding simulation-aware comments to verify the workflow without physical execution.

Yes, Opentrons Protocol API v2 supports custom tip management during liquid handling by allowing you to define tip strategy patterns for transfers, distributions, and consolidations.

Your Opentrons protocol requires a run(context) function because Protocol API v2 uses this entry point to execute automation commands, load labware, and control modules during the robot's operation.