orfverifier

Verify plasmid ORFs by running a six-frame translation against your provided amino acid sequences. The workflow cross-checks translated DNA against reference proteins, enforcing strict matching and reporting strand, frame, and start/end positions.

Yes, you can use an NCBI accession for on-demand verification and annotation. The tool accepts direct FASTA or GenBank input for plasmid sequences and performs optional accession lookups to fetch the reference proteins needed for validation.

Six-frame translation validates protein-coding regions by translating plasmid DNA in all six reading frames to identify potential ORFs. It cross-checks these translated sequences against provided amino acid references to confirm the exact presence and location of coding regions.

ORF verification works directly with FASTA files, GenBank files, or NCBI accession numbers. You provide the plasmid sequence input alongside the target amino acid sequence, and the tool handles the translation and matching process automatically.

You can get JSON output by running the verification workflow with the appropriate output format option. The tool generates JSON results containing strand, frame, and start/end positions, allowing seamless integration with downstream bioinformatics pipelines and reporting systems.

If an amino acid sequence is not found, the strict matching workflow will report no valid ORF match for that reference. Ensure your plasmid sequence input is correct and that the reference protein sequence accurately reflects the expected cloned insert.