plannotate-plasmid-annotation

Plasmid annotation identifies functional elements like promoters, terminators, and resistance genes within DNA sequences. This process uses BLAST against curated databases to verify synthetic constructs and generate annotated GenBank files or interactive HTML maps.

Yes, you can use FASTA or raw sequence input for automated plasmid annotation. The tool processes these formats to identify functional elements and correctly handles circular topology, preventing split-feature artifacts in the output maps.

Yes, automated plasmid annotation correctly handles circular topology to prevent split-feature artifacts. This ensures that functional elements spanning the origin are accurately represented in the resulting GenBank files and interactive HTML maps.

Plasmid annotation generates annotated GenBank files, interactive HTML plasmid maps, and CSV feature tables. These outputs are useful for verifying synthetic constructs, preparing submissions, and sharing visual plasmid maps.

Yes, BLAST is required to identify promoters, terminators, resistance genes, origins of replication, tags, and fluorescent proteins. The tool uses BLAST against curated databases to automate the detection of these functional elements.