pathml

Convert whole-slide pathology images into ML-ready datasets with HDF5 storage.

4|Updated Mar 2, 2026
One-click install
npx skills add https://github.com/shushuzn/Rairos --skill pathml-shushuzn
Or copy as Structured Prompt for Agent
Please help me install this Agent Skill.
Skill: pathml
Source: https://github.com/shushuzn/Rairos/tree/main/skills/pathml
Command: npx skills add https://github.com/shushuzn/Rairos --skill pathml-shushuzn

SYSTEM DOCUMENTATION & REQUIREMENTS

💡 This Skill includes references (resource) components.

What problem does it solve?

PathML reduces the time and expertise required to go from whole-slide pathology images to reliable computational outputs by providing end-to-end tooling for loading, preprocessing, segmentation, graph building, and dataset storage.

Core Features & Use Cases

  • Whole-slide image loading (160+ formats): Read WSI pyramids and regions of interest across common vendor formats, DICOM, and OME-TIFF.
  • Preprocessing pipelines: Compose transforms for tissue detection, H&E stain normalization, denoising, artifact/white-space labeling, and multiparametric preparations.
  • Nucleus/cell segmentation & quantification: Segment multiparametric imaging (e.g., CODEX/Vectra) using Mesmer-based workflows and quantify marker expression into ML-friendly structures.
  • Spatial graph construction: Convert segmentation results into cell/tissue graphs with connectivity options (kNN, radius, Delaunay, contact) and graph-ready features for GNNs.
  • Multiparametric imaging support (CODEX, Vectra, MERFISH): Handle cycle collapsing, segmentation, marker quantification, and export for downstream single-cell/spatial analysis.
  • Efficient dataset storage: Persist tiles, masks, features, and metadata using HDF5 organization patterns suitable for batch ML training.

Quick Start

Use the PathML skill to load a whole-slide image, generate tiles, run a pipeline that performs tissue detection followed by H&E stain normalization, and then access the produced tissue mask and processed tiles.

Frequently Asked Questions about pathml

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I convert whole-slide pathology images into ML-ready datasets?

Whole-slide pathology images are converted into ML-ready datasets by orchestrating loading, preprocessing, segmentation, and graph construction into configurable pipelines that output HDF5-backed tiles, masks, and features for batch ML training.

Can I perform H&E stain normalization and tissue detection in a single preprocessing pipeline?

Yes, H&E stain normalization and tissue detection can be composed into a single preprocessing pipeline. You can configure transforms to sequentially handle tissue masking, stain normalization, denoising, and artifact labeling on whole-slide images.

Does this approach support multiplex imaging formats like CODEX, Vectra, and MERFISH?

Yes, multiplex imaging workflows support CODEX, Vectra, and MERFISH formats. The process handles cycle collapsing, nucleus/cell segmentation using Mesmer-based workflows, and marker quantification for downstream spatial analysis.

What is the best way to build spatial graphs from cell segmentation results for GNNs?

Building spatial graphs from cell segmentation results involves converting segmented cells into graph representations using kNN, radius, Delaunay, or contact connectivity options, generating graph-ready features suitable for downstream graph neural networks.

What whole-slide image formats can I load for computational pathology preprocessing?

You can load whole-slide images across 160+ common vendor formats, DICOM, and OME-TIFF. This allows you to read WSI pyramids and regions of interest seamlessly for computational pathology preprocessing and analysis.