spatial-transcriptomics-tutorials-with-omicverse

Execute OmicVerse spatial transcriptomics workflows from preprocessing to downstream analysis.

32|5|Updated Mar 12, 2026
One-click install
npx skills add https://github.com/omicverse/omicclaw --skill spatial-transcriptomics-tutorials-with-omicverse-omicverse
Or copy as Structured Prompt for Agent
Please help me install this Agent Skill.
Skill: spatial-transcriptomics-tutorials-with-omicverse
Source: https://github.com/omicverse/omicclaw/tree/main/src/omicverse_skills/skills/spatial-tutorials
Command: npx skills add https://github.com/omicverse/omicclaw --skill spatial-transcriptomics-tutorials-with-omicverse-omicverse

SYSTEM DOCUMENTATION & REQUIREMENTS

What problem does it solve?

Spatial transcriptomics analyses involve complex, multi-stage workflows from data preprocessing to deconvolution and downstream interpretation. This skill provides a structured tutorial that guides researchers through practical, reproducible steps using OmicVerse.

Core Features & Use Cases

  • End-to-end spatial workflows: Preprocessing (crop, rotate, align), deconvolution (Tangram, cell2location, Starfysh), and downstream analyses (clustering, integration, trajectory, and communication).
  • Defensive validation and best practices to ensure spatial coordinates exist and are numeric before analyses.
  • Reproducible tutorials and API usage examples to apply to Visium, Stereo-seq, and Slide-seq datasets.

Quick Start

Follow the three-stage spatial tutorial to preprocess a Visium dataset, perform cell-type deconvolution, and explore downstream analyses.

Frequently Asked Questions about spatial-transcriptomics-tutorials-with-omicverse

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I perform spatial transcriptomics deconvolution on Visium data using Tangram or cell2location?

You can perform spatial transcriptomics deconvolution on Visium data through OmicVerse tutorials that validate spatial coordinates and apply Tangram or cell2location to estimate cell type abundances across tissue spots.

What is the best way to preprocess Stereo-seq spatial transcriptomics data for downstream analysis?

Preprocessing Stereo-seq spatial transcriptomics data is handled by OmicVerse workflows that crop, rotate, and align spatial coordinates before validating numeric types for downstream clustering and trajectory analyses.

Can I use OmicVerse spatial transcriptomics workflows for Slide-seq datasets?

OmicVerse spatial transcriptomics workflows support Slide-seq datasets across preprocessing, deconvolution, and downstream analyses, requiring validation that spatial coordinates exist and are numeric before executing analytical steps.

How does spatial transcriptomics integration work across multiple tissue sections?

Spatial transcriptomics integration across multiple tissue sections uses STAligner, GraphST, and SpaceFlow within OmicVerse to align spatial coordinates and resolve batch effects for unified downstream clustering.

Do I need to validate spatial coordinates before running spatial transcriptomics clustering?

Validating spatial coordinates before running spatial transcriptomics clustering is required to ensure coordinates exist and are numeric, preventing execution errors during downstream STAGATE clustering and trajectory analyses.

What downstream analyses are available for spatial transcriptomics data after deconvolution?

Downstream analyses available for spatial transcriptomics data after deconvolution include clustering, integration, trajectory inference, and cell communication, applied to Visium, Stereo-seq, and Slide-seq datasets within OmicVerse.