brenda-database

Retrieve and parse enzyme kinetic data from the BRENDA SOAP API.

94|11|Updated Mar 26, 2026
One-click install
npx skills add https://github.com/swaruplab/operon --skill brenda-database-swaruplab
Or copy as Structured Prompt for Agent
Please help me install this Agent Skill.
Skill: brenda-database
Source: https://github.com/swaruplab/operon/tree/main/src-tauri/protocols/brenda-database
Command: npx skills add https://github.com/swaruplab/operon --skill brenda-database-swaruplab

SYSTEM DOCUMENTATION & REQUIREMENTS

💡 This Skill requires zeep, requests, pandas, brenda_client, and includes scripts (resource) and references (resource) components.

What problem does it solve?

Access to comprehensive enzyme data is often scattered across sources; this Skill centralizes retrieval of kinetic parameters (Km, kcat), reaction equations, substrate specificities, and organism-specific enzyme data from the BRENDA SOAP API, enabling researchers to quickly assemble comparative insights.

Core Features & Use Cases

  • Retrieve Km, kcat values and environmental parameters across organisms for a given EC number.
  • Retrieve and parse reaction equations and substrate/inhibitor/cofactor information for pathway analysis.
  • Compare enzyme properties across organisms and export results for modeling and reporting.
  • Use in metabolic engineering workflows to identify suitable enzymes and design retrosynthetic routes.

Quick Start

Query Km values for EC 1.1.1.1 across organisms and generate a comparative report.

Frequently Asked Questions about brenda-database

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I retrieve Km and kcat values for a specific enzyme across multiple organisms?

To retrieve Km and kcat values across organisms, query the BRENDA SOAP API using a specific EC number. The Skill parses the kinetic parameters, applies organism-specific filtering, and exports the structured data for comparative analysis.

Can I extract reaction equations and substrate specificity data from BRENDA for pathway analysis?

Yes, you can extract reaction equations and substrate specificity data from BRENDA. The Skill retrieves and parses substrate, inhibitor, and cofactor information, providing structured outputs suitable for metabolic pathway analysis.

What is the best way to compare enzyme kinetic parameters for cross-organism studies?

The best way to compare enzyme kinetic parameters for cross-organism studies is to centralize BRENDA API retrieval. This Skill aggregates Km, kcat, and environmental parameters, handling rate-limiting and parsing to generate comparative reports.

Do I need Python and pandas to query enzyme data from the BRENDA SOAP API?

Yes, you need Python with pandas, zeep, and requests installed to query the BRENDA SOAP API. These dependencies handle the SOAP client communication, data parsing, and tabular export required for downstream kinetic modeling.

How do I get organism-specific enzyme information for metabolic engineering workflows?

You get organism-specific enzyme information for metabolic engineering by querying BRENDA with an EC number. The Skill extracts organism-specific properties and exports the data, enabling you to identify suitable enzymes and design retrosynthetic routes.

Are there limitations when querying large datasets of enzyme kinetic data from BRENDA?

Limitations when querying large enzyme kinetic datasets from BRENDA include API rate limits and parsing complexities. The Skill mitigates these by implementing built-in rate-limiting and error handling to ensure stable data extraction.