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opentrons-integration

Create Opentrons Protocol API v2 workflows for lab automation robots.

Updated Jul 1, 2026
Search Tags:#pipetting#thermocycler#lab automation#opentrons#protocol api#pcr setup#serial dilution
One-click install
npx skills add https://github.com/jasrajtulsi/GRAD-SCOPE --skill opentrons-integration-jasrajtulsi
Or copy as Structured Prompt for Agent▼
Please help me install this Agent Skill.
Skill: opentrons-integration
Source: https://github.com/jasrajtulsi/GRAD-SCOPE/tree/main/.claude/skills/opentrons-integration
Command: npx skills add https://github.com/jasrajtulsi/GRAD-SCOPE --skill opentrons-integration-jasrajtulsi

SYSTEM DOCUMENTATION & REQUIREMENTS

💡 This Skill requires opentrons, and includes scripts (resource) and references (resource) components.

What problem does it solve?

This Skill removes the friction of authoring Opentrons robot protocols by giving you a ready-to-use guide for labware setup, pipetting logic, and module control in Python.

Core Features & Use Cases

  • Protocol Authoring: Create Opentrons Protocol API v2 workflows with the correct metadata, run function structure, and execution pattern.
  • Liquid Handling and Hardware Control: Load pipettes, labware, adapters, and modules while managing transfers, distributions, consolidations, and temperature, magnetic, heater-shaker, and thermocycler operations.
  • Practical Workflow Templates: Adapt the included serial dilution, PCR setup, and basic transfer templates for common automation tasks on Flex and OT-2 robots.
  • Use Case: A lab automation engineer can generate a complete PCR setup protocol that loads reagents, distributes master mix, adds samples, and runs thermocycler cycling with simulation-friendly structure.

Quick Start

Ask for a complete Opentrons Flex protocol that transfers liquid from one well to another and includes the necessary metadata, labware, and instrument setup.

Frequently Asked Questions about opentrons-integration

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I write an Opentrons protocol for PCR setup and thermocycler control?▼

Opentrons protocol creation for PCR setup involves structuring Protocol API v2 workflows with correct metadata, loading labware and pipettes, distributing master mix, adding samples, and executing thermocycler cycling commands.

What is the correct structure for an Opentrons Protocol API v2 workflow?▼

An Opentrons Protocol API v2 workflow requires valid protocol metadata, a defined run function, API-level compatible commands, and valid liquid-handling and hardware-control steps to ensure successful robot execution on Flex and OT-2 platforms.

Can I use Opentrons modules like the heater-shaker and thermocycler in my lab automation protocol?▼

Opentrons protocols support module control for temperature, magnetic, heater-shaker, and thermocycler operations, allowing you to load adapters and manage hardware alongside liquid transfers, distributions, and consolidations.

How do I automate serial dilution workflows on an Opentrons Flex or OT-2 robot?▼

Automating serial dilution on Opentrons robots utilizes Protocol API v2 templates that define liquid handling logic, load necessary pipettes and labware, and structure transfer steps to achieve accurate simulation-ready automated dilutions.

Does Opentrons protocol simulation require specific hardware-control steps?▼

Opentrons simulation requires valid liquid-handling and hardware-control steps structured within the protocol, ensuring the API-level commands and module operations are compatible with the robot execution environment.

What's the best way to structure Opentrons labware setup and pipette loading in Python?▼

Structuring Opentrons labware setup and pipette loading requires defining the correct protocol metadata and using Protocol API v2 commands to declare instruments, adapters, and labware positions for automated liquid handling.

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