opentrons-integration

Create and execute Opentrons Protocol API v2 protocols for OT-2 and Flex robots.

4|1|Updated Jun 18, 2025
One-click install
npx skills add https://github.com/HolobiomicsLab/Toolomics --skill opentrons-integration-holobiomicslab
Or copy as Structured Prompt for Agent
Please help me install this Agent Skill.
Skill: opentrons-integration
Source: https://github.com/HolobiomicsLab/Toolomics/tree/main/mcp_host/skills/scientific-skills/scientific-skills/opentrons-integration
Command: npx skills add https://github.com/HolobiomicsLab/Toolomics --skill opentrons-integration-holobiomicslab

SYSTEM DOCUMENTATION & REQUIREMENTS

💡 This Skill requires opentrons, and includes scripts (resource) and references (resource) components.

What problem does it solve?

Opentrons Protocol API v2 enables developers to write and run automated laboratory protocols for OT-2 and Flex robots, centralizing protocol logic and hardware control.

Core Features & Use Cases

  • Protocol structure and metadata: define metadata, requirements, and the run function to execute experiments.
  • Hardware and module integration: load labware, instruments, and modules (temperature, magnetic, heater-shaker, thermocycler, absorbance reader).
  • Advanced liquid handling patterns: transfer, distribute, consolidate, mix, and flow-rate control for precise liquid handling across decks.
  • Typical use cases include PCR setup, serial dilutions, instrument control, and complex multi-module workflows.

Quick Start

Use the Opentrons integration templates to initialize a protocol, load devices, and perform a simple transfer between wells.

Frequently Asked Questions about opentrons-integration

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I automate Opentrons protocols using API v2?

Automating Opentrons protocols with API v2 involves defining metadata, loading labware and modules, and implementing liquid-handling operations like transfer, distribute, and mix within a run function.

Can I integrate temperature, magnetic, and heater-shaker modules in Opentrons protocols?

Yes, Opentrons protocol automation supports loading and controlling temperature, magnetic, heater-shaker, thermocycler, and absorbance reader modules across OT-2 and Flex robot decks.

What is the best way to structure liquid-handling workflows for PCR setup and serial dilutions?

Use Opentrons API v2 templates to initialize protocols, load devices, and apply advanced liquid handling patterns like consolidate and flow-rate control for PCR setup and serial dilutions.

Does Opentrons API v2 support both OT-2 and Flex robots for protocol design?

Yes, Opentrons API v2 enables developers to write and run automated laboratory protocols designed for both OT-2 and Flex robots, centralizing protocol logic and hardware control.

How do I specify protocol metadata and requirements in Opentrons API v2?

Specify protocol metadata and requirements by defining the necessary structures before the run function to execute experiments on OT-2 and Flex robots using Opentrons API v2.

Why are my Opentrons liquid-handling operations failing across multiple modules?

Failures in Opentrons liquid-handling operations often stem from incorrect labware loading or improper module integration, which API v2 protocol automation templates help structure correctly.